A highly sensitive and reliable detection of CA15-3 in patient plasma with electrochemical biosensor labeled with magnetic beads

Biosensors & Bioelectronics
Sattar Akbari NakhjavaniYadollah Omidi

Abstract

An early on-time detection of breast cancer can effectively affect the outcome of the treatment. Here, we developed an ultrasensitive, simple and reliable immunosensor to detect the lowest alteration of CA 15-3, the standard biomarker of breast cancer patients. The proposed immunosensor was achieved by modification of gold electrode by streptavidin to immobilize the biotinylated anti-CA 15-3 monoclonal antibody (mAb). Bovine serum albumin was used to prevent nonspecific binding. To improve the sensitivity of modified immunosensor, the sandwich signal enhancer consisting of streptavidin-coated magnetic beads conjugated with biotinylated horseradish peroxidase (HRP) and anti-CA 15-3 biotinylated mAb was applied. The electrochemical measurements were obtained in the presence of hydroquinone as a redox agent and H2O2 as the activating agent of HRP. Under optimized condition and using square wave voltammetry, the lower limit of quantification was obtained as 15 × 10-6 U/mL and the linear CA 15-3 concentration range was 50-15 × 10-6 U/mL. While showing significant stability, the immunosensor displayed an excellent sensitivity and specificity for the detection of CA 15-3 even in the human serum as compared to the enzyme-linked immunos...Continue Reading

Citations

Nov 22, 2019·Mikrochimica Acta·Ramin PourakbariBalal Khalilzadeh
Mar 3, 2020·Journal of Drug Targeting·Siamak AlizadehYadollah Omidi
Jul 8, 2020·Analytical and Bioanalytical Chemistry·Na LiYiping Cui
Jul 24, 2020·Sensors·Veronika GajdosovaJan Tkac
Jan 29, 2020·Biosensors & Bioelectronics·Parvin Samadi PakchinYadollah Omidi
Mar 5, 2021·Biosensors & Bioelectronics·Veronika VanovaVojtech Adam
Oct 31, 2020·International Journal of Biological Macromolecules·Zahra KarimzadehBalal Khalilzadeh
Apr 12, 2021·Talanta·Erkan YilmazMustafa Soylak
May 15, 2019·International Journal of Biological Macromolecules·Balal KhalilzadehMohammad-Reza Rashidi
Nov 10, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Wenxiao HuYafei Dong
Aug 18, 2021·Journal, Genetic Engineering & Biotechnology·Rola NadeemMahmoud M Bahgat

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