A highly sensitive and specific system for large-scale gene expression profiling

BMC Genomics
Guohong HuHonghua Li

Abstract

Rapid progress in the field of gene expression-based molecular network integration has generated strong demand on enhancing the sensitivity and data accuracy of experimental systems. To meet the need, a high-throughput gene profiling system of high specificity and sensitivity has been developed. By using specially designed primers, the new system amplifies sequences in neighboring exons separated by big introns so that mRNA sequences may be effectively discriminated from other highly related sequences including their genes, unprocessed transcripts, pseudogenes and pseudogene transcripts. Probes used for microarray detection consist of sequences in the two neighboring exons amplified by the primers. In conjunction with a newly developed high-throughput multiplex amplification system and highly simplified experimental procedures, the system can be used to analyze >1,000 mRNA species in a single assay. It may also be used for gene expression profiling of very few (n = 100) or single cells. Highly reproducible results were obtained from duplicate samples with the same number of cells, and from those with a small number (100) and a large number (10,000) of cells. The specificity of the system was demonstrated by comparing results fr...Continue Reading

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Citations

Aug 1, 2015·Journal of Chemical Information and Modeling·Weizhuang ZhouRuss B Altman
Jul 30, 2014·Parasitology·Andrew P Jackson

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Datasets Mentioned

BETA
GSE5920

Methods Mentioned

BETA
biopsy
electrophoresis
PCR
PCRs

Software Mentioned

GeneChip
Kodak
BLAST
BLAT
Genepix Pro

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