PMID: 6402564Mar 1, 1983Paper

A highly sensitive enzyme immunoassay for mouse beta nerve growth factor

Journal of Neurochemistry
S FurukawaK Hayashi

Abstract

A sensitive two-site enzyme immunoassay system for mouse beta nerve growth factor (NGF) was developed, based on the sandwiching of the antigen between anti-mouse beta NGF antibody IgG coated to a polystyrene tube and anti-mouse beta NGF antibody Fab'-linked beta-D-galactosidase (beta-D-galactoside hydrolase, EC 3.2.1.23). This method has the following advantages: (a) the procedures are simple and rapid compared to bioassay or two-site radioimmunoassay; (b) antibody Fab'-beta-D-galactosidase complex is more stable than 125I-labeled antibody; (c) purified beta NGF is detectable at a concentration as low as 10 pg/ml. Our enzyme immunoassay was used to examine the levels of NGF in some tissues of mice. The submaxillary gland contained a high concentration of NGF. However, other tissues, such as the heart, brain, and skeletal muscle, and serum did not contain detectable NGF. These results support recent findings by other investigators that NGF was not found in the organs/tissues other than the submaxillary gland of mice.

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Citations

Jul 9, 1999·Journal of Neuroscience Research·A NittaS Furukawa
Sep 5, 2002·Journal of Neuroscience Research·Hisanori ItoShoei Furukawa
Apr 1, 1993·Journal of Neuroscience Research·H AwatsujiK Hayashi
Jul 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·T JohR Tanaka
Jun 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·K MuraseK Hayashi
Feb 26, 1987·Journal of Immunological Methods·L Lärkfors, T Ebendal
Aug 1, 1989·Journal of the Neurological Sciences·N SunoharaE Satoyoshi
Jun 1, 1985·Brain Research·S J KanakisD J Watters
Sep 30, 1987·Biochemical and Biophysical Research Communications·S FurukawaK Hayashi
Jun 1, 2002·Journal of the Neurological Sciences·Yutaka MurakamiYoshiko Furukawa
Apr 26, 2006·Bioscience, Biotechnology, and Biochemistry·Noriko HattoriShoei Furukawa
Jun 1, 1993·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·M Näher-NoéB Conrad
Nov 3, 2015·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Shoei Furukawa
Mar 1, 1986·Journal of Neurochemistry·J Lakshmanan
Mar 1, 1984·Clinics in Endocrinology and Metabolism·W H Daughaday, E Heath

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