PMID: 3383814May 1, 1988Paper

A highly sensitive gas chromatographic method does not detect exhalation of volatile hydrocarbons from isolated ventilated lungs under massive peroxidative stress

Experimental Lung Research
W SeegerH Neuhof

Abstract

Lung lipid peroxidation is thought to be a basic pathophysiological phenomenon responsible for pulmonary damage in different types of oxidant attack. Measurement of volatile hydrocarbons, especially ethane and pentane, produced during peroxidative degradation of polyunsaturated fatty acids and exhaled into an animal housing chamber, has attracted increasing interest for the monitoring of in vivo lipid peroxidation. However, this approach cannot distinguish between pulmonary exhalation of hydrocarbons generated in different organs or even the intestinum and pulmonary generation of these lipid peroxidation markers. In the present study we developed a respiration and hydrocarbon trapping system for isolated, ventilated, and perfused lungs that avoided rebreathing and allowed complete sampling and gas chromatographic separation and quantification of exhaled alkanes and alkenes (C1-C5) in the absence of background levels. Using an "artificial lung," the recoveries of exogenously administered hydrocarbons ranged between 80 and 95% with good reproducibility (SD between 1.7 and 9.6%). The detection limit of the system was approximately 3 fmol of each alkane or alkene/g wet lung weight.min. However, neither under basal conditions nor du...Continue Reading

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Citations

Jan 1, 1991·Free Radical Biology & Medicine·W A Pryor, S S Godber
Jan 1, 1991·Free Radical Biology & Medicine·C K Chow
Aug 1, 1994·Free Radical Biology & Medicine·C M KneepkensC C Roy
Jan 1, 1989·Free Radical Research Communications·S DuttaJ W Thayer
Jan 1, 1993·Free Radical Research Communications·A Cailleux, P Allain
Jan 1, 1995·Experimental Lung Research·M P Habib, N C Clements

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