A highly versatile automatized setup for quantitative measurements of PHIP enhancements

Journal of Magnetic Resonance
Alexey S KiryutinGerd Buntkowsky

Abstract

The design and application of a versatile and inexpensive experimental extension to NMR spectrometers is described that allows to carry out highly reproducible PHIP experiments directly in the NMR sample tube, i.e. under PASADENA condition, followed by the detection of the NMR spectra of hyperpolarized products with high spectral resolution. Employing this high resolution it is feasible to study kinetic processes in the solution with high accuracy. As a practical example the dissolution of hydrogen gas in the liquid and the PHIP kinetics during the hydrogenation reaction of Fmoc-O-propargyl-l-tyrosine in acetone-d6 are monitored. The timing of the setup is fully controlled by the pulse-programmer of the NMR spectrometer. By flushing with an inert gas it is possible to efficiently quench the hydrogenation reaction in a controlled fashion and to detect the relaxation of hyperpolarization without a background reaction. The proposed design makes it possible to carry out PHIP experiments in an automatic mode and reliably determine the enhancement of polarized signals.

Citations

Sep 24, 2020·The Journal of Chemical Physics·Vitaly P KozinenkoKonstantin L Ivanov
Sep 4, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Andreas B SchmidtJan-Bernd Hövener
Jan 3, 2020·The Journal of Chemical Physics·Sara HadjialiGerd Buntkowsky
Jan 3, 2019·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Alexey S KiryutinGeoffrey Bodenhausen
Dec 7, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ludmilla GuduffGaspard Huber
Oct 16, 2019·Physical Chemistry Chemical Physics : PCCP·Sergey KorchakStefan Glöggler
Jan 31, 2019·Chemistry : a European Journal·Alexey S KiryutinGerd Buntkowsky
Feb 3, 2021·Magma·Francesca ReineriSilvio Aime
May 20, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Alexey S KiryutinKonstantin L Ivanov
Feb 27, 2021·Angewandte Chemie·Andrey N PravdivtsevJan-Bernd Hövener

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