A histidine variant of yeast iso-1-cytochrome c that strongly affects the energetics of the denatured state

Journal of Molecular Biology
S Godbole, B E Bowler

Abstract

Iso-1-cytochrome c has been engineered to remove all histidine residues not involved in heme ligation in the native state to produce a variant designated TM. Single histidine residues were then introduced at positions 26, TM + His26, and 54, TM + His54. Since histidine residues not involved in native state heme ligation are known to replace the methionine 80 heme ligand in denatured cytochrome c, these variants were expected to affect the structure of the denatured state. Guanidine hydrochloride denaturations were performed to assess the stability of these proteins relative to the wild-type protein. The free energy difference for heme ligation in the denatured state was assessed by pH titration. The experimentally observed mutation-induced change (delta deltaG(D-state)) in the free energy of heme ligation for unfolded TM + His54 versus TM + His26 is -0.4 kcal/mol. The expected mutation-induced change in delta deltaG(D-state) calculated for a random coil unfolded state is +2 kcal/mol. Thus, unfolded TM + His54 has residual structure stabilizing its denatured state by -2.4 kcal/mol relative to TM + His26. The results imply that the denatured state can contribute significantly to mutation-induced changes in the free energy of unfo...Continue Reading

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Citations

Jul 28, 1999·Protein Science : a Publication of the Protein Society·P Wittung-Stafshede
May 5, 2004·Journal of Molecular Biology·Eydiejo Wandschneider, Bruce E Bowler
Sep 28, 2005·Journal of Molecular Biology·Eydiejo KurchanBruce E Bowler
Oct 31, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rastislav VarhacMikulás Bánó
Jan 25, 2007·Molecular BioSystems·Bruce E Bowler
Feb 6, 2018·Biophysical Journal·Travis A Danielson, Bruce E Bowler
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May 11, 2006·Chemical Reviews·Zhengshuang ShiNeville R Kallenbach
Mar 24, 2004·Biochemistry·Rastislav Varhac, Marián Antalík
Jul 10, 2012·Biophysical Journal·Michaela L Finnegan, Bruce E Bowler

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