PMID: 2567Dec 8, 1975

A histochemical study of the apparent deamination of proteins by sodium hypochlorite

Histochemistry
P J Stoward

Abstract

The possible chemical mechanisms by which neutral solutions of sodium hypochlorite containing a high concentration of sodium chloride abolish the acidophilia of proteins in sections of fixed tissue are reviewed. The most probable one is the chlorination of the protein terminal amino groups, followed by the breakdown of the N-chloramine so formed into alpha-ketocarboxylic acid, nitrile or aldehyde groups. Hypochlorite solutions certainly do not deaminate tissue sections as was previously thought. Experimental evidence for the formation of relatively stable N-chloramine groups in situ and their limited conversion to aldehydes is reported. For example, the acidophilia of hypochlorite-treated sections was found to be restored after flooding them with hydriodic acid followed by the extraction of the liberated iodine with an alcohol. The significance of these experimental findings is discussed.

References

Jan 1, 1968·Histochemie. Histochemistry. Histochimie·P J StowardM Plant
Jan 1, 1955·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S BURSTONE
Nov 1, 1963·Journal of Chromatography· VON ARX, R NEHER
Oct 1, 1953·Proceedings of the National Academy of Sciences of the United States of America·M Alfert, I I Geschwind
Jun 1, 1916·The Biochemical Journal·H D Dakin

Citations

Oct 1, 1995·European Journal of Oral Sciences·C Sismanidou, S Lindskog
Jul 30, 2010·Dental Materials Journal·Florian Just WegehauptThomas Attin
Oct 30, 2007·Journal of Endodontics·M MarendingM Zehnder
Aug 19, 2007·International Endodontic Journal·M MarendingM Zehnder
Jan 17, 2009·International Dental Journal·Z Mohammadi
Mar 3, 2017·Chemical Reviews·Alideertu DongGe Gao
Sep 15, 2017·BioMed Research International·Tariq S Abuhaimed, Ensanya A Abou Neel
Aug 9, 2017·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Tian-Feng WangTao Jiang

Related Concepts

Aldehydes
Metazoa
Chloramines
Deamination
Glycosaminoglycans
Cytochemistry
Hydrogen-Ion Concentration
Gene Products, Protein
Clorox
Mouse, Swiss

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