A HPLC-MS/MS method for determination of 6'''-feruloylspinosin in rat plasma and tissues: Pharmacokinetics and tissue distribution study

Journal of Pharmaceutical and Biomedical Analysis
Longdong QiaoWenlin Xie

Abstract

A sensitive, reliable and accurate HPLC-MS/MS method was developed and validated for the quantification of 6'''-feruloylspinosin in rat plasma and tissues with puerarin as the internal standard. The separation was performed on a Proshell 120 EC-C18 column (4.6×150 mm, 2.7 μm) with a mobile phase consisting of acetonitrile and 0.1% formic acid (20:80, v/v) at 0.3 mL/min. The quantification was performed by MRM with m/z [M-H](-) 783.3→427.2 for 6'''-feruloylspinosin and m/z [M-H](-) 415.4→295.4 for the internal standard, respectively. The calibration curves covered over a concentration range of 20-2000 ng/mL in plasma and various tissues samples (heart, liver, spleen, lung, kidney, stomach, intestine, muscle, cerebrum and cerebellum) with good linearity (r(2)≥0.9914). Both the intra- and inter-day precisions were less than 14.70%, and the accuracy (RE%) ranged from -5.80% to 4.93%. The extraction recoveries were within 75.21-92.96%, and the matrix effect ranged from 87.21% to 113.44%. Compared with spinosin, 6'''-feruloylspinosin was distributed in rats faster whereas more slowly eliminated from the plasma. 6'''-Feruloylspinosin could be distributed rapidly and widely in various tissues, and transfer across the blood-brain barrie...Continue Reading

References

Aug 2, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yu-Juan LiYu-Lin Deng
Oct 7, 2008·Journal of Pharmaceutical and Biomedical Analysis·Yujuan LiShan Cheng
Feb 3, 2012·Journal of Asian Natural Products Research·Lin ZhangDan Yuan
Mar 4, 2014·Pharmacology, Biochemistry, and Behavior·In Ho JungJong Hoon Ryu
Mar 20, 2014·European Journal of Pharmacology·Michelangelo AuteriRosa Serio
Apr 23, 2015·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jonathan FischellScott M Thompson

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