A human bone marrow mesodermal-derived cell population with hemogenic potential

Leukemia
Saloomeh MokhtariGraça Almeida-Porada

Abstract

The presence, within the human bone marrow, of cells with both endothelial and hemogenic potential has been controversial. Herein, we identify, within the human fetal bone marrow, prior to establishment of hematopoiesis, a unique APLNR+, Stro-1+ cell population, co-expressing markers of early mesodermal precursors and/or hemogenic endothelium. In adult marrow, cells expressing similar markers are also found, but at very low frequency. These adult-derived cells can be extensively culture expanded in vitro without loss of potential, they preserve a biased hemogenic transcriptional profile, and, upon in vitro induction with OCT4, assume a hematopoietic phenotype. In vivo, these cells, upon transplantation into a fetal microenvironment, contribute to the vasculature, and generate hematopoietic cells that provide multilineage repopulation upon serial transplantation. The identification of this human somatic cell population provides novel insights into human ontogenetic hematovascular potential, which could lead to a better understanding of, and new target therapies for, malignant and nonmalignant hematologic disorders.

References

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Citations

Apr 8, 2020·Molecular Therapy. Methods & Clinical Development·Nadia El-AkabawyGraça Almeida-Porada

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Methods Mentioned

BETA
flow cytometry
PCR
Fluorescence

Software Mentioned

FluoView
GraphPad Prism

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