A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways.

The Journal of Biological Chemistry
G Fischer von Mollard, T H Stevens

Abstract

Membrane traffic in eukaryotic cells requires the interaction of a vesicle-associated soluble NSF attachment protein receptor (v-SNARE) on transport vesicles with a SNARE on the target membrane (t-SNARE). Recently, we identified the yeast protein Vti1p as a v-SNARE that is involved in two transport reactions. Vti1p interacts with the prevacuolar t-SNARE Pep12p in Golgi to prevacuolar transport and with the cis-Golgi t-SNARE Sed5p in traffic to the cis-Golgi. Here we describe a human Vti1p homolog, hVti1. Whereas vti1Delta cells are inviable, expression of hVti1 allows vti1Delta cells to grow at nearly the wild-type growth rate. When expressed in yeast hVti1 can replace Vti1p in both Golgi to prevacuolar transport and in traffic to the cis-Golgi. Sequence comparisons with a Schizosaccharomyces pombe and two different mouse Vti1 homologs led to the identification of a very conserved predicted alpha-helix. Amino acid exchanges in vti1 mutant alleles defective either in one or both trafficking steps cluster in this domain, suggesting that this structure is probably the binding site for effector proteins.

References

Jan 1, 1977·Genetics·E W Jones
Nov 6, 1992·Science·J M White
Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J ColicelliM Wigler
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V A BankaitisS D Emr
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·D C BasshamN V Raikhel
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·B BergerP S Kim
Nov 3, 1994·Nature·J E Rothman
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Jan 1, 1994·Annual Review of Neuroscience·R Jahn, T C Südhof
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Jan 1, 1993·Cytogenetics and Cell Genetics·A Swaroop, J Xu
Mar 8, 1996·The Journal of Biological Chemistry·J C HayR H Scheller
Feb 14, 1997·The Journal of Biological Chemistry·D FasshauerA T Brünger
Jun 2, 1997·The Journal of Cell Biology·I PaekJ E Rothman
Jul 11, 1997·The Journal of Biological Chemistry·J A McNewT H Söllner
Jul 1, 1997·Molecular Biology of the Cell·J B BockR H Scheller

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Citations

Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Albert S MellickNigel A Morrison
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ajaya J KunwarGabriele Fischer von Mollard
May 15, 2002·Molecular Biology of the Cell·Naïma Belgareh-TouzéRosine Haguenauer-Tsapis
Jun 8, 1999·Molecular Biology of the Cell·G Fischer von Mollard, T H Stevens
Jan 27, 2004·Molecular Biology of the Cell·Regina KuliawatPeter Arvan
Apr 24, 1999·The Plant Cell·A A Sanderfoot, N V Raikhel
Dec 14, 1999·Protein Science : a Publication of the Protein Society·T TishgartenL A Lipscomb
Jul 16, 2003·Molecular and Cellular Biology·Vadim AtlashkinGabriele Fischer von Mollard
Jun 29, 2000·Annual Review of Biochemistry·R Jahn, T C Südhof
Oct 25, 2013·Molecular Biology of the Cell·Jason V RogersMark D Rose
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maite SanmartínEnrique Rojo
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D FasshauerR Jahn
Oct 25, 2006·Molecular Membrane Biology·Ya Wang, Bor Luen Tang
Mar 1, 2007·Expert Review of Clinical Immunology·Marta Hoffman-Sommer, Joanna Rytka
Dec 31, 2003·The Journal of Cell Biology·Oleg VarlamovJames E Rothman
Jul 1, 2010·Journal of Cellular Physiology·Yanan ChenBor Luen Tang
Feb 27, 1999·Experimental Cell Research·H R Pelham
Dec 30, 2014·Frontiers in Cellular Neuroscience·Sven Truckenbrodt, Silvio O Rizzoli
Jun 7, 2014·The EMBO Journal·Alexander M WalterJakob B Sørensen
Jul 11, 2001·The Journal of Biological Chemistry·M DilcherG F von Mollard
Aug 26, 2018·Nature Communications·Javier Emperador-MeleroMatthijs Verhage
Nov 25, 2003·The Journal of Biological Chemistry·Subbulakshmi ChidambaramGabriele Fischer von Mollard
Nov 1, 2020·Life Sciences·Cheryl Qian Ying Yong, Bor Luen Tang
Jun 18, 2002·European Journal of Cell Biology·Vera KreykenbohmGabriele Fischer von Mollard

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