PMID: 7021592Aug 1, 1981Paper

A human neutrophil-dependent pathway for generation of angiotensin II. Purification of the product and identification as angiotensin II

The Journal of Clinical Investigation
B U WintroubK W Watt

Abstract

A human neutrophil lysosomal protease interacts at physiologic pH with a 62,000--67,000-mol wt plasma protein substrate to generate a vasoactive, smooth muscle-contracting "neutral" peptide. The peptide product of this system, previously designated the "neutral" peptide-generating pathway, was generated from purified components and purified by Bio-Gel P2 gel filtration and reverse-phase high performance liquid chromatography with a 50--60% yield of starting activity. The purified peptide had an amino acid composition of Asx, Pro, Val, Ile, Tyr, Phe, His, Arg, a composition identical to that of angiotensin II. The peptide and synthetic angiotensin II each filtered at 48--52% bed volume on Bio-Gel P2, had an isoelectric point of Ph 7.8--8.1 at 4 degrees C, migrated 3 cm toward the cathode during pH 6.4 low-voltage paper electrophoresis, and had a retention time of 44.8 min during reverse-phase high-performance liquid chromatography. In addition, the functional activity of the peptide at each purification step correlated with angiotensin II content determined by specific radioimmunoassay. The amino acid sequence of 25 nmol of the peptide was Asp-Arg-Val-Try-Ile-His-Pro-Phe, the same covalent structure as that of angiotensin II. Th...Continue Reading

References

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Citations

Dec 19, 2001·Archives of Biochemistry and Biophysics·Ahmed Ramaha, Philip A Patston
Jul 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·J R IngelfingerV J Dzau
Dec 2, 1999·Molecular and Cellular Endocrinology·P S LeungC Sernia
Apr 5, 2003·The International Journal of Biochemistry & Cell Biology·Yusuke SuzukiJesus Egido
Sep 6, 2000·Clinical and Experimental Pharmacology & Physiology·S TakaiM Miyazaki
Nov 14, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Y ItoK Ohyashiki
Jan 1, 1982·The Journal of Clinical Investigation·M G TonnesenB U Wintroub
Jan 1, 1983·Clinical and Experimental Hypertension. Part A, Theory and Practice·J GenestP Hamet
Mar 11, 1988·The American Journal of Medicine·A SantucciF Balsano
Jul 15, 1993·The American Journal of Cardiology·Y UrabeK Arakawa
Aug 18, 2006·Journal of Hypertension·Jana RyklHartmut Schlüter
Nov 20, 1998·Journal of Cardiovascular Pharmacology·S TakaiM Miyazaki
Jun 12, 2013·Molecular Medicine Reports·Hiroyuki FurukawaShoichi Iseki
Nov 1, 1984·The Journal of Investigative Dermatology·B U WintroubL B Schwartz

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