PMID: 9432525Feb 12, 1998Paper

A human recombinant erythropoietin produced in human lymphoblastoid cells

Journal de pharmacie de Belgique
A Bounemra-Ben GhanemK Boukef

Abstract

A human recombinant erythropoietin is produced by transfection of a human EBV immortalised lymphoblastoid cell line by human erythropoietin gene. This cell line was selected because it is eucaryotic, human, able to grow in suspension and neither clonogenic nor tumorigenic. Purification of erythropoietin from culture supernatants of lymphoblastoid cells was described and structural, biochemical and functional characteristics were investigated in several studies. Results obtained for this kind of recombinant erythropoietin were similar to those obtained regarding the protein expressed in CHO cells suggesting that both molecules have the same efficiency in clinical applications.

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.