A hyaluronic acid binding peptide-polymer system for treating osteoarthritis

Biomaterials
Heather J FaustJennifer H Elisseeff

Abstract

Hyaluronic acid (HA) is found naturally in synovial fluid and is utilized therapeutically to treat osteoarthritis (OA). Here, we employed a peptide-polymer cartilage coating platform to localize HA to the cartilage surface for the purpose of treating post traumatic osteoarthritis. The objective of this study was to increase efficacy of the peptide-polymer platform in reducing OA progression in a mouse model of post-traumatic OA without exogenous HA supplementation. The peptide-polymer is composed of an HA-binding peptide (HABP) conjugated to a heterobifunctional poly (ethylene glycol) (PEG) chain and a collagen binding peptide (COLBP). We created a library of different peptide-polymers and characterized their HA binding properties in vitro using quartz crystal microbalance (QCM-D) and isothermal calorimetry (ITC). The peptide polymers were further tested in vivo in an anterior cruciate ligament transection (ACLT) murine model of post traumatic OA. The peptide-polymer with the highest affinity to HA as tested by QCM-D (∼4-fold greater binding compared to other peptides tested) and by ITC (∼3.8-fold) was HABP2-8-arm PEG-COLBP. Biotin tagging demonstrated that HABP2-8-arm PEG-COLBP localizes to both cartilage defects and synovium....Continue Reading

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Citations

Feb 27, 2020·Expert Opinion on Pharmacotherapy·Peihua CaoDavid J Hunter
Feb 11, 2021·Pharmaceuticals·Oliver McClurgLinda Troeberg
Jan 19, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Deborah MasonFiona E Watt
Mar 29, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura AlderferDonny Hanjaya-Putra
May 1, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhi-Wei HuangQing Yao
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jun XueHui Zhou
Sep 19, 2021·Advanced Healthcare Materials·Xiaojie LinMiqin Zhang
Oct 4, 2021·Advanced Healthcare Materials·Anna GilpinShyni Varghese

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