A hybrid baculovirus-T7 RNA polymerase system for recovery of an infectious virus from cDNA

Virology
C C YapY Matsuura

Abstract

We established a hybrid baculovirus-T7 RNA polymerase system for transient expression in mammalian cells. Two recombinant baculoviruses carrying cDNA of bacteriophage T7 RNA polymerase, with or without a nuclear localization signal, under the control of a mammalian promoter were constructed. High level expression of T7 RNA polymerase was observed in various mammalian cell lines after infection with the recombinant baculoviruses. After transfection of plasmids containing the luciferase gene under the control of the T7 promoter, high luciferase activity was detected in cells infected with the recombinant baculoviruses. We also constructed a plasmid containing an entire cDNA clone of type 1 poliovirus under the T7 promoter. Two days after transfection of the plasmid into the cells infected with the recombinant baculoviruses, a high titer of poliovirus was recovered. The use of the recombinant baculoviruses did not cause any cytopathic effects even at a high multiplicity of infection. The lack of replication ability and low toxicity are the advantageous features of the hybrid baculovirus-T7 polymerase system in comparison with the widely used vaccinia-T7 polymerase system for gene expression and recovery of infectious viruses from ...Continue Reading

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O Elroy-Stein, B Moss
Nov 1, 1990·Nature·B MossT R Fuerst
Nov 11, 1989·Nucleic Acids Research·A LieberM Strauss
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G KaplanV R Racaniello
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S van der WerfJ J Dunn
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Nov 20, 1981·Science·V R Racaniello, D Baltimore
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P DavanlooF W Studier
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·C HofmannM Strauss
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P WhelanG T Wertz
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·F M Boyce, N L Bucher
Mar 1, 1995·Bio/technology·F van PoelwijkR W Goldbach

❮ Previous
Next ❯

Citations

May 7, 2005·Nature Biotechnology·Thomas A KostDonald L Jarvis
Jun 2, 2001·Human Gene Therapy·L PieroniN La Monica
Aug 28, 2003·Journal of Virology·Hideki TaniYoshiharu Matsuura
Jul 29, 2004·Journal of Virology·Andrea FacciabeneNicola La Monica
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P CondreayT A Kost
Dec 19, 2003·Protein Expression and Purification·Mark A MillerMichael A Whitt
Apr 29, 2015·Viruses·Kaisa-Emilia MakkonenSeppo Ylä-Herttulala
Nov 30, 2006·Protein Expression and Purification·Martin J ScottSteve L Martin
Jan 25, 2005·Journal of Biotechnology·Serafín GutiérrezMiguel López-Ferber
Nov 30, 2004·Virology·Anice C LowenRichard M Elliott
Jul 4, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sudip GhoshSeyed E Hasnain
Nov 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sari P KukkonenSeppo Ylä-Herttuala
Sep 23, 2000·Gene Therapy·K J AirenneS Ylä-Herttuala
Feb 1, 2003·The Journal of General Virology·Leslie L Domier, Nancy K McCoppin
Jun 21, 2018·Molecular Genetics and Genomics : MGG·Hao ZhengJingchen Sun
Mar 3, 2010·Cold Spring Harbor Protocols·Anna R MäkeläChristian Oker-Blom

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.