PMID: 8970963Dec 1, 1996Paper

A hybrid herpesvirus infectious vector based on Epstein-Barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo

Journal of Virology
S Wang, J M Vos

Abstract

We have developed a miniviral vector, pH300, based on the human herpesviruses 1 and 4, herpes simplex virus type 1 (HSV-1), and Epstein-Barr virus (EBV), carrying EBV sequences for plasmid episomal maintenance and HSV-1 sequences for amplification and packaging in multimeric form into HSV-1 capsids in the presence of a helper virus and helper cell line. A reporter gene, the bacterial lacZ gene, which expressed beta-galactosidase, was inserted into the multiple cloning site of pH300 to make pH300-lac. The packaged pH300-lac DNA was very efficient in infecting human cells in tissue culture. The pH300-lac miniviral stock was used to infect in vitro various human cell types derived from breast cancer, lung cancer, and liver cancer. Up to 95% of cells were infected and expressed beta-galactosidase activity after exposure to viral stock at a multiplicity of infection of 3. There was essentially no apparent cytotoxicity after infection of cultured cells in vitro. To test in vivo gene delivery, human liver tumor cells preimplanted subcutaneously in nude mice and injected in situ with pH300-lac showed high efficiency of ectopic gene expression. The pH300 miniviral vector is a simple and effective gene transfer system which shows potenti...Continue Reading

References

May 1, 1992·Journal of Virology·P A JohnsonT Friedmann
May 8, 1992·Science·W F Anderson
Jun 11, 1992·Nature·A D Miller
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A I Geller, A Freese
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A I GellerA B Pardee
Sep 1, 1989·Microbiological Reviews·J G Stevens
Jul 1, 1988·The Journal of General Virology·D J McGeochP Taylor
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R R Spaete, N Frenkel
Jun 11, 1987·Nucleic Acids Research·N A DeLuca, P A Schaffer
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R D Cone, R C Mulligan
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A Vlazny, N Frenkel
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J YatesB Sugden
May 1, 1993·Trends in Biotechnology·T Friedmann, H A Jinnah
May 14, 1993·Science·R C Mulligan

❮ Previous
Next ❯

Citations

May 3, 2003·Nature Reviews. Neuroscience·Beverly L Davidson, Xandra O Breakefield
Jun 24, 2009·Memórias do Instituto Oswaldo Cruz·Alberto Luis Epstein
Aug 6, 1999·Gene Therapy·N J CaplenW J Ramsey
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·H J DelecluseW Hammerschmidt
Aug 7, 2003·Endothelium : Journal of Endothelial Cell Research·Raffaella Dell'EvaDouglas Noonan
Oct 19, 2005·Gene Therapy·Alberto L Epstein
Feb 24, 2001·The Journal of Gene Medicine·P Y Lam, X O Breakefield
Apr 23, 2008·Current Protocols in Human Genetics·Andrew Braun
Apr 23, 2008·Current Protocols in Neuroscience·R L Neve, F Lim
Dec 4, 2009·Viruses·Suresh de Silva, William J Bowers
Jan 5, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·S Connelly, M Kaleko
Mar 28, 2002·Human Gene Therapy·Gary HsichXandra O Breakefield
Apr 23, 2008·Current Protocols in Human Genetics·Cornel Fraefel
Jun 15, 2005·Annual Review of Biochemistry·Inder M Verma, Matthew D Weitzman
Apr 23, 2008·Current Protocols in Neuroscience·Cornel Fraefel
Aug 2, 2007·Expert Opinion on Biological Therapy·Delphine CuchetAlberto L Epstein
Oct 12, 2013·PloS One·Ralf WeiskirchenCarsten Bergmann
Sep 19, 2000·Journal of Neurogenetics·D J LimD E Weinstein
Feb 16, 2000·Gene Therapy·L C CostantiniO Isacson
Jul 4, 2012·Current Protocols in Neuroscience·Andrea S Laimbacher, Cornel Fraefel
Aug 8, 1998·Journal of Virology·T A Stavropoulos, C A Strathdee
Jan 25, 2018·Current Protocols in Human Genetics·Scott Swindle
Oct 7, 2005·BioTechniques·Rachael L NeveWilliam A Carlezon
Jun 10, 1998·Biochemical and Biophysical Research Communications·W J RamseyR M Blaese
Jul 19, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·M Sena-EstevesX O Breakefield
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·H LebloisM Perricaudet
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·Y SaekiE A Chiocca
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·W J BowersH J Federoff
May 29, 2000·Trends in Genetics : TIG·W BruneU H Koszinowski
Apr 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Richard Wade-MartinsE Antonio Chiocca
Sep 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Angelika OehmigXandra O Breakefield
Oct 29, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olivia C HibbittRichard Wade-Martins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.