A hybridization-chain-reaction-based method for amplifying immunosignals

Nature Methods
Rui LinMinmin Luo

Abstract

Immunosignal hybridization chain reaction (isHCR) combines antibody-antigen interactions with hybridization chain reaction (HCR) technology, which results in amplification of immunofluorescence signals by up to two to three orders of magnitude with low background. isHCR's highly modular and easily adaptable design enables the technique to be applied broadly, and we further optimized its use in multiplexed imaging and in state-of-the-art tissue expansion and clearing techniques.

References

Dec 20, 1989·Journal of Immunological Methods·M N BobrowG J Litt
Dec 7, 2002·Nature Biotechnology·Antje KepplerKai Johnsson
Sep 25, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Neenu VargheseC N R Rao
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
May 29, 2009·Angewandte Chemie·Chun-Hua LuGuo-Nan Chen
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bijan ZakeriMark Howarth
Mar 25, 2014·Neuron·Zhixiang LiuMinmin Luo
Oct 17, 2014·Nature·Yong ShiQiusheng Gu
Nov 5, 2014·Nature Methods·Peter C FridyMichael P Rout
Dec 17, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Daniel E Carvajal-HausdorfDavid L Rimm
Jan 17, 2015·Science·Fei ChenEdward S Boyden
Jan 27, 2015·Nature Cell Biology·Subramanian P RamanathanDaniel J Muller
Apr 29, 2015·Nature Methods·Sarada ViswanathanLoren L Looger
Jul 18, 2015·Cell·Douglas S Richardson, Jeff W Lichtman
Feb 13, 2016·Cell·Emily Lauren SylwestrakKarl Deisseroth
Apr 12, 2016·Nature Methods·Tyler J ChozinskiJoshua C Vaughan
May 31, 2016·Cell·Nicolas RenierMarc Tessier-Lavigne
Aug 23, 2016·Nature Methods·Chenchen PanAli Ertürk
Feb 18, 2017·Genetics·Ben SutcliffeSebastian Cachero
Apr 19, 2017·Nature Methods·Jae-Byum ChangEdward S Boyden
Apr 30, 2017·Chemical Science·Sarit S AgastiPeng Yin

❮ Previous
Next ❯

Citations

Sep 5, 2018·Lab on a Chip·Alexander M XuJames R Heath
Aug 21, 2019·Nature Biotechnology·Sinem K SakaPeng Yin
Feb 15, 2020·Chemical Communications : Chem Comm·Xiaojing XingWeihong Tan
Dec 24, 2018·Nature Methods·Asmamaw T WassieEdward S Boyden
Nov 26, 2019·Advanced Science·Han-Eol ParkSung-Yon Kim
Aug 14, 2019·Chemical Society Reviews·Wenjing WangJun-Jie Zhu
Dec 9, 2020·Nature Methods·De-En SunXing Chen
Dec 10, 2020·Science China. Chemistry·Yongxi ZhaoChunhai Fan
Nov 10, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yusuke KitamuraToshihiro Ihara
Apr 5, 2021·Neurobiology of Disease·Brendan R Gallagher, Yongxin Zhao
May 20, 2021·Journal of Agricultural and Food Chemistry·Panting SangHe Qian
Aug 24, 2021·Journal of the American Chemical Society·Gang WenJohan Hofkens
Jul 11, 2018·Journal of the American Chemical Society·Wouter EngelenMaarten Merkx
Aug 28, 2021·Journal of the American Chemical Society·Kunihiko MorihiroAkimitsu Okamoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal for Immunotherapy of Cancer
Edward C StackPeter P Lee
Biochimica Et Biophysica Acta
V A NAJJAR, J FISHER
© 2022 Meta ULC. All rights reserved