A hydrogel-based enzyme-loaded polymersome reactor

Nanoscale
Hans-Peter M de HoogRoeland J M Nolte

Abstract

In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A 'continuous-flow polymersome reactor' is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarctica lipase B (CALB) and glucose oxidase (GOx).

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Citations

Dec 14, 2011·ACS Applied Materials & Interfaces·Bo PengFangqiong Tang
Jan 2, 2013·ACS Applied Materials & Interfaces·Xianwei MengFangqiong Tang
Mar 27, 2012·Inorganic Chemistry·Mausam KalitaStefan H Bossmann
Dec 30, 2015·International Journal of Biological Macromolecules·Cevahir AltinkaynakNalan Özdemir
Mar 15, 2016·Frontiers in Bioengineering and Biotechnology·Christine SchmittLars M Voll
May 17, 2012·Proteomics·Vivienne N LukAaron R Wheeler
Mar 20, 2016·Enzyme and Microbial Technology·Burcu SomturkIsmail Ocsoy
Oct 18, 2012·Chemical Society Reviews·Maïté MarguetSébastien Lecommandoux
Dec 25, 2013·Chemical Society Reviews·Matthieu RaynalPiet W N M van Leeuwen
Jul 17, 2016·Zoology : Analysis of Complex Systems, ZACS·Adhityo WicaksonoParvez Alam
Oct 8, 2016·Chemical Reviews·Gonzalo PrietoFerdi Schüth
Feb 23, 2020·Angewandte Chemie·Jianbo LiuStephen Mann
Aug 31, 2020·Advanced Healthcare Materials·Loredana LeggioNunzio Iraci
Aug 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Karolina LangowskaWolfgang Meier
May 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·R P PogorilyiV G Kessler

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