A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers

International Journal of Nanomedicine
Liping SunJian Weng

Abstract

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD. A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrPC) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor. Electrochemical impedance spectroscopy was utilized for AβO analysis. The specific binding between AβO and PrPC probes on the hydrogel electrode resulted in an increase in the electron-transfer resistance. The biosensor showed high specificity and sensitivity for AβO detection. It could selectively differentiate AβO from amyloid-beta (Aβ) monomers or fibrils. Meanwhile, it was highly sensitive to detect as low as 0.1 pM AβO in artificial CSF or blood plasma. The linear range for AβO detection is from 0.1 pM to 10 nM. This biosensor could be used as a cost-effective tool for early diagnosis of AD due to its high electrochemical performance and bionic structure.

Citations

Jul 23, 2019·Critical Reviews in Clinical Laboratory Sciences·Peter D Fransquet, Joanne Ryan
Mar 8, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Haoran HuQiang Wang
Jan 25, 2019·Current Medicinal Chemistry·Weslley Felix de OliveiraMaria Tereza Dos Santos Correia
Oct 31, 2019·Journal of Analytical Methods in Chemistry·Tong ZhangShujing Li
Feb 27, 2020·Scientific Reports·Masoud SoltanRezaee, Mahdi Bodaghi
May 8, 2020·Toxicology Mechanisms and Methods·Jayabal PrakashGopinath Devanand Venkatasubbu
Apr 21, 2020·Journal of Alzheimer's Disease : JAD·Young Chul YounSangYun Kim
Aug 17, 2019·Current Medicinal Chemistry·Javad TavakoliYouhong Tang
Feb 15, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yilan XuHeng Zhang
Mar 30, 2021·International Journal of Nanomedicine·Zhengguo QiuXu Li
May 1, 2021·Nanomaterials·Le Minh Tu PhanSungbo Cho

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Methods Mentioned

BETA
circular dichroism
enzyme-linked immunosorbent assay
biosensors
biosensor
biosensing
X-ray
AFM
imaging technique
Fluorescence

Software Mentioned

JWSSE

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