PMID: 6170933Aug 25, 1981Paper

A hydrogen exchange study of Escherichia coli 5S RNA

Nucleic Acids Research
J Ramstein, V A Erdmann

Abstract

Using the tritium Sephadex method, the number of exchangeable protons in E. coli 5S RNA and the kinetics of their exchange reactions have been measured at two different Mg++ concentrations (10(-2) M and 10(-3) M). A quantitative analysis of these results indicates the presence of two classes of protons exchanging with very different rates. The protons of the slow class, not seen in linear molecules of double helical RNA, exchange with a half-time of 0.6 hour and their exchange kinetics are independent of Mg++ concentration. Reduction of the Mg++ concentration from 10(-2) ot 10(-3) M, however, results in a decrease in the number of these exchangeable protons form 26 to 19. Neither the total number, nor the exchange kinetics of the fast protons are affected by this change in Mg++ concentration. Comparison of these results with those previously obtained with tRNA, suggests the presence of a Mg++ dependent tertiary structure in 5S RNA. The number of exchangeable protons obtained from extrapolation of the exchange curves (120 and 126 respectively for 10(-3) and 10(-2) M Mg++ concentration) are compared to the calculated number of exchangeable protons predicted by previous proposed structural models for E.Coli 5S RNA.

References

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Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J Ramstein, R H Buckingham

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Citations

Feb 1, 1990·International Journal of Biological Macromolecules·H Ishigaki, S Sugai
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Pieler, V A Erdmann
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·V A ErdmannT Pieler
Aug 25, 1982·Nucleic Acids Research·M J Kime, P B Moore
Mar 7, 1983·FEBS Letters·M J Kime, P B Moore
Nov 16, 1982·Biochemical and Biophysical Research Communications·D S Tewari, D P Burma

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