A hydroponics based high throughput screening system for Phytophthora root rot resistance in chickpea (Cicer arietinum L.)

Plant Methods
Amritha AmalrajTim Sutton

Abstract

Phytophthora root rot (PRR) caused by P. medicaginis is a major soil borne disease in chickpea growing regions of Australia. Sources of resistance have been identified in both cultivated and wild Cicer species. However, the molecular basis underlying PRR resistance is not known. Current phenotyping methods rely on mycelium slurry or oospore inoculum. Sensitive and reliable methods are desirable to study variation for PRR resistance in chickpea and allow for a controlled inoculation process to better capture early defence responses following PRR infection. In this study, a procedure for P. medicaginis zoospore production was standardized and used as the inoculum to develop a hydroponics based in planta infection method to screen chickpea genotypes with established levels of PRR resistance. The efficiency of the system was both qualitatively validated based on observation of characteristic PRR symptom development, and quantitatively validated based on the amount of pathogen DNA in roots. This system was scaled up to screen two biparental mapping populations previously developed for PRR studies. For each of the screenings, plant survival time was measured after inoculation and used to derive Kaplan-Meier estimates of plant surviva...Continue Reading

References

Oct 24, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Arūnas P VerbylaRobin Thompson
Apr 30, 2009·Annual Review of Phytopathology·Dina A St Clair
May 9, 2012·Current Opinion in Infectious Diseases·Despoina GkentziShamez N Ladhani
Nov 20, 2016·Journal of Neuromuscular Diseases·Katherine E VestAnita H Corbett
Sep 10, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Adam NormanHaydn Kuchel
Feb 15, 2018·Nature Communications·Eric J B von WettbergDouglas R Cook
Dec 12, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Amritha AmalrajTim Sutton

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
DNA assay
Pty

Software Mentioned

WGAIM R package
R Statistical Computing Environment ( R Core Team
ASReml
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.