A hyperpolarization-activated, cyclic nucleotide-gated, (Ih-like) cationic current and HCN gene expression in renal inner medullary collecting duct cells

American Journal of Physiology. Cell Physiology
Juan J BolívarElvira Galarraga

Abstract

The cation conductancein primary cultures of rat renal inner medullary collecting duct was studied using perforated-patch and conventional whole cell clamp techniques. Hyperpolarizations beyond -60 mV induced a time-dependent inward nonselective cationic current (I(vti)) that resembles the well-known hyperpolarization-activated, cyclic nucleotide-gated I(h) and I(f) currents. I(vti) showed a half-maximal activation around -102 mV with a slope factor of 25 mV. It had a higher conductance (but, at its reversal potential, not a higher permeability) for K(+) than for Na(+) (gK(+)/gNa(+) = 1.5), was modulated by cAMP and blocked by external Cd(2+) (but not Cs(+) or ZD-7288), and potentiated by a high extracellular K(+) concentration. We explored the expression of the I(h) channel genes (HCN1 to -4) by RT-PCR. The presence of transcripts corresponding to the HCN1, -2, and -4 genes was observed in both the cultured cells and kidney inner medulla. Western blot analysis with HCN2 antibody showed labeling of approximately 90- and approximately 120-kDa proteins in samples from inner medulla and cultured cells. Immunocytochemical analysis of cell cultures and inner medulla showed the presence of HCN immunoreactivity partially colocalized w...Continue Reading

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Citations

Dec 25, 2012·Journal of the American Society of Nephrology : JASN·Karl P RoosDonald E Kohan
Jul 29, 2011·Kidney International·Rolando Carrisoza-GaytánLaura I Escobar
Jan 24, 2012·Biochemical and Biophysical Research Communications·Rania NasrallahRichard L Hébert
Nov 16, 2013·Molecular Biology Reports·Ana I CalejoPaula P Gonçalves
Dec 23, 2011·The Journal of Experimental Biology·Ednan GerardPhilippe Coulon
Aug 18, 2021·Progress in Biophysics and Molecular Biology·Patrizia BenzoniAnnalisa Bucchi

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