A Hypoallergenic Polygalacturonase Isoform from Olive Pollen Is Implicated in Pollen-Pollen Cross-Reactivity

International Archives of Allergy and Immunology
Carmen Oeo-SantosRodrigo Barderas

Abstract

Cross-reactivity reactions between allergenic polygalacturonases (PGs) from different biological sources, especially foods and pollens from the Oleaceae family, have been described using Salsola kali PG (Sal k 6). No PG from olive pollen has been characterized to date, hampering further knowledge about cross-reactions through PGs. The aim of this work was to determine the potential allergenicity of the PG from olive pollen and clarify its role in cross-reactivity. A cDNA-encoding olive pollen PG sequence was subcloned into the pET41b vector and used to transform BL21(DE3) Escherichia coli cells to produce a His-tag fusion recombinant protein. The allergenic properties of olive pollen PG were determined by immunoblotting and ELISA in comparison to Sal k 6. The cross-reactivity potential of the protein with other pollen sources was analyzed by inhibition immunoassays. The existence of other isoforms of Ole e 14 with different allergenicity was confirmed by proteomics and a meta-analysis of the recently reported olive genome. Sal k 6 showed a higher IgE recognition than Ole e 14 regardless of patient sensitization, suggesting the existence of more allergenic Ole e 14 isoforms in olive pollen. IgG and IgE inhibition assays supporte...Continue Reading

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Citations

Jul 22, 2020·Expert Review of Clinical Immunology·Pascal PoncetDenis Charpin
Jun 14, 2019·Journal of Proteome Research·Pablo San Segundo-AcostaRodrigo Barderas
Jan 29, 2020·Molecular Pharmaceutics·Sara BenedéEva Batanero

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