A Japanese encephalitis virus genotype 5 molecular clone is highly neuropathogenic in a mouse model: impact of the structural protein region on virulence

Journal of Virology
Mélissanne de WispelaerePhilippe Desprès

Abstract

Japanese encephalitis virus (JEV) strains can be separated into 5 genotypes (g1 to g5) based on sequence similarity. JEV g5 strains have been rarely isolated and are poorly characterized. We report here the full characterization of a g5 virus generated using a cDNA-based technology and its comparison with a widely studied g3 strain. We did not observe any major differences between those viruses when their infectious cycles were studied in various cell lines in vitro. Interestingly, the JEV g5 strain was highly pathogenic when inoculated to BALB/c mice, which are known to be largely resistant to JEV g3 infection. The study of chimeric viruses between JEV g3 and g5 showed that there was a poor viral clearance of viruses that express JEV g5 structural proteins in BALB/c mice blood, which correlated with viral invasion of the central nervous system and encephalitis. In addition, using an in vitro model of the blood-brain barrier, we were able to show that JEV g5 does not have an enhanced capacity for entering the central nervous system, compared to JEV g3. Overall, in addition to providing a first characterization of the understudied JEV g5, our work highlights the importance of sustaining an early viremia in the development of JEV...Continue Reading

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Citations

Jan 14, 2017·PLoS Neglected Tropical Diseases·Mélissanne de WispelaereValérie Choumet
Mar 24, 2017·Journal of Neuroinflammation·Rafael Elias MarquesMauro M Teixeira
Jun 8, 2018·The Journal of General Virology·Kuniaki NeromeReiko Nerome
Dec 15, 2015·Journal of Virology·Mélissanne de WispelaereNathalie Pardigon
May 3, 2018·Journal of Travel Medicine·James C PearceJames G Logan
Oct 6, 2015·PLoS Neglected Tropical Diseases·Mélissanne de WispelaerePhilippe Desprès
Nov 6, 2018·Viruses·Ginés Ávila-PérezLuis Martínez-Sobrido
Dec 4, 2020·International Journal of Molecular Sciences·Lise FiacreGaëlle Gonzalez

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