Abstract
Serine protease inhibitors (SPIs) are an important group of protease inhibitors involved in a variety of biological processes. In the present study, a Kazal-type serine protease inhibitor homolog gene (designated as CsKPI) was identified from a Cyclina sinensis cDNA library. The open reading frame consists of 456 bp and encodes a protein of 151 amino acid residues with a theoretical molecular mass of 16.85 kDa and an isoelectric point of 5.74. Furthermore, using quantitative real-time PCR, we focused on the expression patterns of CsKPI found in tissues and on the stimulation of this gene's expression by bacteria. The results show that a higher-level mRNA expression of CsKPI was detected in hemocytes (P < 0.05) and was significantly upregulated at 3 h (P < 0.01) upon receiving bacterial challenges with Vibrio anguillarum. In addition, after the CsKPI gene was silenced by RNA interference, the expression of the CsTLR2 and CsMyD88 genes was extremely significantly decreased (P < 0.01) in C. sinensis. Finally, the recombinant CsKPI (rCsKPI) protein was purified and shown to exhibit less inhibitory activity than C-lyz against V. anguillarum in vitro. Hence, we propose that CsKPI plays an important role in the innate immunity and med...Continue Reading
References
Aug 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M M KrahnD C Malins
Jan 1, 1980·Annual Review of Biochemistry·M Laskowski, I Kato
Mar 17, 2000·Immunological Reviews·R Medzhitov, C Janeway
Apr 18, 2000·Molecular Genetics and Metabolism·P LeandroD Konecki
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Dec 19, 2003·Nucleic Acids Research·Ivica LetunicPeer Bork
Jun 18, 2004·Immunological Reviews·Lage Cerenius, Kenneth Söderhäll
Apr 13, 2005·Journal of Biotechnology·Tanja Pascale NeuviansRainer Grobholz
Oct 17, 2006·Journal of Virology·Haipeng LiuKenneth Söderhäll
Oct 28, 2006·The Journal of Biological Chemistry·Sang Woon ShinAlexander S Raikhel
Mar 21, 2008·Fish & Shellfish Immunology·Bo WangKezhi Xing
Jul 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·S Tariq AhmadFen-Biao Gao
Dec 9, 2009·The FEBS Journal·Chunju AnMichael R Kanost
Jul 14, 2010·Insect Biochemistry and Molecular Biology·Chunju An, Michael R Kanost
Sep 21, 2010·Biochimie·David GubbAne Fullaondo
Jan 11, 2011·Fish & Shellfish Immunology·Haiyang YuXiming Guo
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Apr 10, 2013·Fish & Shellfish Immunology·Lingling WangLinsheng Song
Nov 12, 2013·Developmental and Comparative Immunology·Yongjie LiuXiaolin Liu
Feb 7, 2015·Fish & Shellfish Immunology·Zhuang XueCenjie Liu