A keratinocyte and integrated fibroblast culture model for studying particulate matter-induced skin lesions and therapeutic intervention of fucosterol

Life Sciences
I P Shanura FernandoYou-Jin Jeon

Abstract

Increased levels of particulate matter (PM) air pollutants in East Asia have resulted in detrimental health impacts increasing morbidity and mortality. Epidemiological studies suggest a possible relation between the cutaneous exposure of PM and increased oxidative stress and inflammation which lead to skin lesions. The present study utilizes an integrated cell culture model of keratinocytes and fibroblasts to mimic viable skin layers and investigate the possible effects of PM exposure after penetration through corneocytes. The skin perfection is upheld by homeostatic functionality of epidermal cells and the integrity of connective tissues. Exposure to xenobiotics could alter the skin cell homeostasis aggravating premature skin aging. Stimulation of HaCaT keratinocytes by PM collected from Beijing, China (CPM) increased the intracellular ROS levels triggering a cascade of events aggravating inflammatory responses and connective tissue degradation. In HDF fibroblasts, treatment with preconditioned keratinocyte culture media augmented inflammatory responses, cellular differentiation, and connective tissue degradation. Above events were marked by the increased intracellular ROS, inflammatory mediators, pro-inflammatory cytokines, m...Continue Reading

References

May 17, 2000·The Science of the Total Environment·R M Harrison, J Yin
Feb 24, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·C J Miller, G H Stein
Apr 17, 2007·The Journal of Investigative Dermatology·Sabine WernerHans Smola
Apr 17, 2008·Analytical and Bioanalytical Chemistry·Ikuko MoriMasataka Nishikawa
Dec 18, 2013·Lancet·Zhu ChenYan-Shen Zhang
Oct 29, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Natalia D MagnaniGiuseppe Valacchi
Jul 12, 2017·International Journal of Biological Macromolecules·I P Shanura FernandoYou-Jin Jeon

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Citations

Mar 11, 2020·Polymers·Andrea Catalina Villamil BallesterosYuli Alexandra Neira Bejarano
Jul 28, 2020·Particle and Fibre Toxicology·Irini M DijkhoffBarbara Rothen-Rutishauser
Nov 22, 2020·International Journal of Biological Macromolecules·Ilekuttige Priyan Shanura FernandoGinnae Ahn
Apr 11, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Md Abdul HannanIl Soo Moon
Sep 22, 2020·Biomolecules & Therapeutics·Youn Jin KimJae Sung Hwang
Jul 1, 2020·International Journal of Biological Macromolecules·Ilekuttige Priyan Shanura FernandoGinnae Ahn
Jul 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ping DiaoLi Li
Aug 20, 2021·Photodermatology, Photoimmunology & Photomedicine·Soomin KimJu Hee Lee

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