PMID: 7525734Nov 1, 1994Paper

A keratinocyte cell line synthesizes a predominant insulin-like growth factor-binding protein (IGFBP-3) that modulates insulin-like growth factor-I action

The Journal of Investigative Dermatology
C J WraightG A Werther

Abstract

Insulin-like growth factor-I (IGF-I) is an important regulator of epidermal proliferation and has been shown in vitro to be a powerful stimulator of keratinocyte growth. It is synthesized by fibroblasts in the dermis, along with several IGF-binding proteins (IGFBPs), which are known to modulate IGF-I responsiveness of virtually all tissues studied. Because it was not known how or in what form IGF-I produced in the dermis acts on epidermal keratinocytes in vivo, we investigated the possible role of IGFBPs in modulating the response of epidermal keratinocytes to IGF-1. We show here that tIGF-I, a non-IGFBP-binding analogue of IGF-1, is a more potent mitogenic stimulator of the keratinocyte cell line HaCaT than IGF-I, suggesting that keratinocytes produce IGFBPs that modulate their response to IGF-I. To confirm this and to identify which IGFBPs were produced, we analyzed HaCaT cell-conditioned medium and mRNA, with the following findings: HaCaT cells produce a major IGFBP, identified as IGFBP-3, and a minor 24-kD IGFBP, likely to be IGFBP-4. Northern analysis revealed a 2.6-kb IGFBP-3 mRNA; however, IGFBP-4 mRNA was not detectable. We conclude that production of predominantly IGFBP-3 by the HaCaT cell line modulates its sensitivit...Continue Reading

References

Jan 1, 1991·The Journal of Investigative Dermatology·E K NeelyR G Rosenfeld
Nov 1, 1991·The Journal of Investigative Dermatology·G A Scott, A R Haake
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R L Eckert, H Green
May 1, 1993·The Journal of Investigative Dermatology·Y Ando, P J Jensen

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Citations

Jul 22, 2010·International Journal of Cosmetic Science·Y Sugiyama-NakagiriT Kitahara
Jan 28, 2012·Anais Brasileiros De Dermatologia·Guilherme Póvoa, Lucia Martins Diniz
May 14, 1999·The Journal of Clinical Endocrinology and Metabolism·S R EdmondsonG A Werther
Dec 19, 2003·Expert Opinion on Biological Therapy·P J WhiteC J Wraight
Jun 23, 2010·Archives of Dermatological Research·Yoriko Sugiyama-NakagiriTakashi Kitahara
Sep 24, 2010·Journal of Periodontal Research·Y TakenouchiK Ito
Dec 13, 2003·Endocrine Reviews·Stephanie R EdmondsonChristopher J Wraight
Oct 8, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Munenori TakaokaHiroshi Nakagawa
Dec 19, 2006·Molecular Cancer Therapeutics·Yaguang XiJingfang Ju
Aug 12, 2004·The Journal of Investigative Dermatology·Hye Jung Kim, Tae-Yoon Kim
Nov 22, 2005·The Journal of Investigative Dermatology·Nicole Weger, Thomas Schlake

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