A key role for telomerase reverse transcriptase unit in modulating human embryonic stem cell proliferation, cell cycle dynamics, and in vitro differentiation.

Stem Cells
Chunbo YangM Lako

Abstract

Embryonic stem cells (ESC) are a unique cell population with the ability to self-renew and differentiate into all three germ layers. Human ESC express the telomerase reverse transcriptase (TERT) gene and the telomerase RNA (TR) and show telomerase activity, but TERT, TR, and telomerase are all downregulated during the differentiation process. To examine the role of telomerase in human ESC self-renewal and differentiation, we modulated the expression of TERT. Upregulation of TERT and increased telomerase activity enhanced the proliferation and colony-forming ability of human ESC, as well as increasing the S phase of the cell cycle at the expense of a reduced G1 phase. Upregulation of TERT expression was associated with increases in CYCLIN D1 and CDC6 expression, as well as hyperphosphorylation of RB. The differentiated progeny of control ESC showed shortening of telomeric DNA as a result of loss of telomerase activity. In contrast, the differentiated cells from TERT-overexpressing ESC maintained high telomerase activity and accumulated lower concentrations of peroxides than wild-type cells, implying greater resistance to oxidative stress. Although the TERT-overexpressing human ESC are able to form teratoma composed of three germ...Continue Reading

References

Jun 4, 1998·Current Opinion in Genetics & Development·J Lingner, T R Cech
Mar 6, 1999·The Journal of Biological Chemistry·W FuM P Mattson
Sep 30, 1999·Nature Medicine·W C HahnR A Weinberg
Sep 27, 2001·Cell·E H Blackburn
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Steven E ArtandiRonald A DePinho
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sheila A StewartRobert A Weinberg
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peisu ZhangMark P Mattson
Mar 26, 2003·Nature Genetics·Sheila A StewartRobert A Weinberg
Apr 26, 2003·Cloning and Stem Cells·M K CarpenterM S Rao
Apr 30, 2003·Nature Cell Biology·Laura L SmithJames M Roberts
Jul 31, 2003·Cell·Kenkichi MasutomiWilliam C Hahn
Sep 6, 2003·Current Biology : CB·Hiroyuki TakaiTitia de Lange
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Miodrag StojkovicAlison Murdoch
Nov 24, 2004·Nature Structural & Molecular Biology·Ming LeiThomas R Cech
Jul 15, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Federico CalegariWieland B Huttner
Jul 23, 2005·Science·Ignacio FloresMaría A Blasco

❮ Previous
Next ❯

Citations

Jan 31, 2013·The Journal of Biological Chemistry·Hae-Yun JungJae-Il Park
Nov 6, 2009·Carcinogenesis·Steven E Artandi, Ronald A DePinho
Dec 3, 2009·Molecular Human Reproduction·Dean H Betts, Bill Kalionis
May 4, 2011·Molecular and Cellular Biology·Angela Ying-Jian LiDavid Kong Ann
Apr 22, 2014·Stem Cell Reports·Hoi-Hung CheungOwen M Rennert
Jan 19, 2010·Annual Review of Pathology·Douglas C WallaceVincent Procaccio
Apr 23, 2014·Stem Cells and Development·Lida RadanDean Harvey Betts
Jul 22, 2014·The International Journal of Biochemistry & Cell Biology·Chunyi LiChris McMahon
Oct 16, 2012·Stem Cell Reviews and Reports·Fernando P HartwigFlávio F Demarco
Apr 25, 2013·The Journal of Biological Chemistry·Xin WangJae-Il Park
Jun 10, 2015·Rejuvenation Research·Jasmin S NurkovicMiodrag Stojkovic
Feb 25, 2014·Protein & Cell·Yan HuangZhou Songyang
May 16, 2014·Science China. Life Sciences·Feng KongDaWei Xu
Apr 7, 2010·Ageing Research Reviews·Jun-Ping LiuHe Li
Feb 9, 2010·Molecular and Cellular Endocrinology·Lucy CassarJun-Ping Liu
Oct 3, 2009·Mitochondrion·Douglas C Wallace, Weiwei Fan
May 22, 2009·Experimental Gerontology·Gabriele Saretzki
Oct 15, 2009·International Endodontic Journal·L T FriedlanderR M Love
Mar 30, 2012·Birth Defects Research. Part C, Embryo Today : Reviews·Chunyi Li
Nov 6, 2009·Journal of Cellular Physiology·Klaus A BeckerGary S Stein
Sep 18, 2009·Journal of Cellular Biochemistry·Jamie F Conklin, Julien Sage
Jul 22, 2015·ELife·Kunitoshi ChibaDirk Hockemeyer
Mar 3, 2009·Experimental Gerontology·Jedrzej HoffmannIoakim Spyridopoulos
Dec 19, 2012·Archives of Oral Biology·Alejandro Iglesias-LinaresAsunción Mendoza-Mendoza
Oct 10, 2014·PloS One·Dolores Bautista-EspañaPatricia Sánchez-Alonso
Jan 22, 2011·Progress in Neurobiology·Helena L A VieiraAlessandro Vercelli
Dec 17, 2016·Human Reproduction Update·D K HapangamaG Saretzki

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