A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based Dental Implants

Cells, Tissues, Organs
Milena R KaluđerovićSanja Mijatović

Abstract

Autophagy plays an important role in embryogenesis, for the maintenance of tissue homeostasis and the elimination of damaged subcellular structures. Furthermore, autophagy could be a mode of physiological cell death and also be implicated in cell differentiation. Thus, we hypothesized that autophagy may have an impact on the differentiation of osteoblast cells influenced by various titanium-based surfaces. Interactions between smooth, commercially available pure titanium (Ti cp), rough Ticer, acid-etched Ti cp (SS) and M1-M3 (comprised of the monoclinic phase of sodium-titanium oxides and rutile; M2 contains amorphous calcium phosphates) and human osteoblast cells were investigated. Immunofluorescent staining was used for detecting autophagy, cell cluster formation and collagen type I (Col-1) expression. Flow cytometry was employed to identify autophagy, the production of endogenous nitric oxide (NO) and the size and granularity of the cells. Rough surfaces caused osteoblast differentiation via the autophagic-dependent PI3/Akt signalling pathway. These surfaces induced the formation of discrete populations of large, granular cells, i.e. mature osteoblasts. In addition, M1-M3 provoked the development of a third population of sma...Continue Reading

Citations

Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Milena R KaluđerovićHans-Ludwig Graf
Nov 16, 2018·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Wookyeom YangJae-Hoon Kim
Nov 14, 2017·Critical Reviews in Toxicology·Donatella GranchiNicola Baldini
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Li ZhangKoroush Kabir
Apr 23, 2020·International Journal of Molecular Sciences·Viviana di GiacomoSilvia Sancilio
Nov 29, 2020·Journal of Periodontal Research·Yuhui YangWeiran Li

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