A kinetic analysis of biosynthesis and localization of a lysosome-associated membrane glycoprotein

Archives of Biochemistry and Biophysics
M P D'Souza, J T August

Abstract

The biosynthesis and subcellular distribution of a major lysosomal membrane glycoprotein of mouse embryo 3T3 cells, LAMP-1, have been examined by [35S]methionine pulse-labeling, sucrose density gradient fractionation, and oligosaccharide analysis. Mature LAMP-1, immunoprecipitated after labeling for 4 h, had a molecular mass of about 110,000 Da. It comigrated during sucrose density fractionation with lysosomal markers, consistent with previous electron microscopic evidence for its localization in lysosomal membranes. Precursor molecules, pulse-labeled for 5 min and extracted during the first 15 min of post-translational processing, were concentrated in the rough endoplasmic reticulum fraction as a species of 92,000 Da. Within 30 min after synthesis, LAMP-1 was found in fractions enriched in Golgi and lysosomal marker enzyme activities as the mature 110,000-Da glycoprotein. Oligosaccharide processing was complete by 1 h after synthesis, and the mature glycoprotein remained in a fraction bearing lysosomal markers. Treatment of the 92,000-Da precursor with endo-beta-N-acetyl-glucosaminidase H produced a core polypeptide of 43,000 Da. Pulse-labeling in the presence of tunicamycin yielded a 42,000-Da form of LAMP-1, which was conver...Continue Reading

References

Jul 8, 1975·Biochemical and Biophysical Research Communications·J S Tkacz, O Lampen
Jul 1, 1985·The Journal of Cell Biology·J W ChenJ T August
May 1, 1986·The Journal of Cell Biology·J Lippincott-Schwartz, D M Fambrough
Dec 1, 1985·The Journal of Cell Biology·H J GeuzeK von Figura
Jun 1, 1985·The Journal of Cell Biology·V LewisI Mellman
Aug 1, 1985·Cell·W G Dunphy, J E Rothman
Oct 1, 1969·The Journal of Cell Biology·B FleischerH Ozawa
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jul 1, 1983·The Journal of Cell Biology·W G Dunphy, J E Rothman
Jan 1, 1983·The Journal of Cell Biology·A L HubbardA Ma
Jul 1, 1984·The Journal of Cell Biology·W J BrownM G Farquhar
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W G DunphyJ E Rothman
Jul 1, 1983·The Journal of Cell Biology·V GieselmannK Von Figura

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Citations

May 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W Hunziker, H J Geuze
Nov 15, 1989·Biochemical and Biophysical Research Communications·Y NoguchiK Kato
Mar 22, 2002·Molecular Biology of the Cell·Brian A RousJ Paul Luzio
Nov 1, 1992·The Journal of Clinical Investigation·A PittA L Schwartz
Jul 14, 2012·Particle and Fibre Toxicology·Eleonore FröhlichThomas R Pieber
Aug 1, 1992·Archives of Biochemistry and Biophysics·S R Carlsson, M Fukuda
Jan 30, 2008·Current Protocols in Cell Biology·Beat E SchaubJack Rohrer
Jun 6, 1994·FEBS Letters·C Peters, K von Figura
May 1, 1999·Archives of Biochemistry and Biophysics·K FurutaJ T August
May 28, 2004·Cell·Brian A CobbDennis L Kasper
Jan 1, 1989·Archives of Biochemistry and Biophysics·S M ManeJ T August

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