PMID: 6991386Jan 1, 1980Paper

A kinetic analysis of the hydrolysis of synthetic arginine substrates by arginine esterases from the venom of the gabooon adder, Bitis gabonica

Hoppe-Seyler's Zeitschrift für physiologische Chemie
C C Viljoen, D P Botes

Abstract

The kinetics of arginine esterases E-I, E-II and E-III from the venom of Bitis gabonica were investigated. With N alpha-benzoyl-L-arginine ethyl ester as substrate linear competitive inhibition versus L-arginine was observed while ethanol gave rise to S-parabolic I-linear noncompetitive inhibition. Hydrolysis of N alpha-benzoyl-L-arginine-p-nitroanilide was noncompetitively inhibited by p-nitroaniline. Both slopes and intercepts of double reciprocal plots were a linear function of inhibitor concentration. Ethanol gave complex inhibition kinetics which could be interpreted in terms of mixed dead-end and alternate product inhibition (S-parabolic I-hyperbolic noncompetitive inhibition). These results imply an ordered uni-bi as the minimal kinetic mechanism wherein ethanol (or amine when amide is used as substrate) is released first from the enzyme surface, followed by the liberation of arginine. The enzymes are inactivated by phenylmethane sulfonyl fluoride which suggests the presence of an essential serine in the active sites of the enzymes. The enzymes may therefore be classified in the group of serine proteases.

References

Dec 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F FiedlerE Werle
Feb 1, 1974·British Journal of Haematology·N A Marsh, B C Whaler
Nov 1, 1961·Archives of Biochemistry and Biophysics·B F ERLANGERW COHEN
Jan 1, 1965·Annual Review of Biochemistry·M L BENDER, J KEZDY

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Citations

Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·F CloeteJ C Schabort
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·N A Marsh, B C Whaler
Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·M SamelJ Siigur
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·E SiigurJ Siigur
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·S Hyslop, N A Marsh

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