PMID: 3408714May 17, 1988

A kinetic light-scattering study of the binding of wheat germ protein synthesis initiation factor 3 to 40S ribosomal subunits and 80S ribosomes

Biochemistry
D J Goss, D Rounds

Abstract

The rate constants for eucaryotic initiation factor 3 (eIF3) association and dissociation with 40S ribosomal subunits and 80S monosomes have been determined. These rate constants were determined by laser light scattering with unmodified eIF3. The affinity of eIF3 for 40S subunits is about 30-fold greater than for 80S ribosomes. This difference in affinity resides mainly in the association rate constants. Rate constants of 8.8 X 10(7) and 7.3 X 10(6) M-1 s-1 were obtained for eIF3 binding to 40S subunits and 80S ribosomes, respectively. From thermodynamic cycles, the affinity of eIF3-40S subunits for 60S subunits is about 30-fold lower than free 40S subunits for 60S subunits. A calculation shows that under these conditions and assuming simple equilibria, approximately 12% of ribosomal subunits would associate via a reaction of 40S-eIF3 with 60S subunits as opposed to a path where eIF3 dissociates from the 40S subunits prior to association with 60S subunits.

References

Dec 17, 1979·Biochimica Et Biophysica Acta·H Trachsel, T Staehelin

Citations

Dec 13, 2007·Quarterly Reviews of Biophysics·Christopher S Fraser, Jennifer A Doudna
Mar 10, 2001·RNA·J S KieftJennifer A Doudna
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W E TapprichA E Dahlberg
Jun 11, 2004·Annual Review of Biochemistry·Lee D Kapp, Jon R Lorsch
Nov 4, 2008·Journal of Molecular Biology·Michael G AckerJon R Lorsch

Related Concepts

Mathematics
Peptide Initiation Factors
Ribosomal Proteins
Ribosomes
Scattering, Radiation
Eukaryotic Initiation Factor-3, Subunit 7

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