Abstract
Nitric oxide (NO) has been known for a long while to act as an inactivator of the soybean lipoxygenase-1 and cyclooxygenase. More recently, NO was shown to interact also with a mammalian 15-lipoxygenase [Wiesner, R., Rathmann, J., Holzhütter, H. G., Stosser, R., Mader, K., Nolting, H. & Kühn, H. (1996) Nitric oxide oxidises ferrous mammalian lipoxygenases to a pre-activated ferric species, FEBS Lett. 389, 229-232]. In this paper we present a detailed kinetic analysis of the interaction of NO with the 15-lipoxygenase from reticulocytes. Time courses of hydroperoxide formation were monitored after an anaerobic incubation of the enzyme with varying concentrations of NO and for varying length of the incubation period. Owing to the presence of O2 during the enzymatic reaction, the inhibitory effect of NO declines in a time-dependent manner since NO is converted into non-reactive NO2. This is manifested as a lag phase of the time course. The lag phase becomes more pronounced if the enzyme is incubated over a fixed period (15 s) with increasing concentrations of NO. In contrast, increasing the length of the incubation period at fixed concentrations of NO diminishes the duration of the lag phase. These experimental findings can be acco...Continue Reading
References
Jun 1, 1979·European Journal of Biochemistry·S M RapoportD Klatt
Jan 23, 1975·Biochimica Et Biophysica Acta·J J de GrootB F van Gelder
Jul 25, 1979·Biochimica Et Biophysica Acta·J C Salerno, J N Siedow
Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto
Aug 25, 1992·Biochemistry·M J SchilstraJ F Vliegenthart
Jan 1, 1992·Lipids·J KannerR Granit
Jan 1, 1991·European Biophysics Journal : EBJ·Y HenryA Guissani
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W RadomskiS Moncada
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J ConradE Sigal
Nov 14, 1991·Nature·D L SloaneE Sigal
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Jun 1, 1990·Acta Physiologica Scandinavica·R F Furchgott
Apr 1, 1989·European Journal of Biochemistry·A HatzelmannV Ullrich
Jan 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L J Ignarro
Sep 4, 1987·Science·B SamuelssonC N Serhan
Nov 16, 1987·European Journal of Biochemistry·A Hatzelmann, V Ullrich
Oct 15, 1987·European Journal of Biochemistry·P LudwigS M Rapoport
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Sneddon, J R Vane
Aug 1, 1982·European Journal of Biochemistry·B HärtelS M Rapoport
Jul 1, 1981·Journal of Inorganic Biochemistry·M P Doyle, J W Hoekstra
Nov 1, 1993·American Journal of Clinical Pathology·F L Kiechle, T Malinski
Jan 1, 1995·Thrombosis Research·Q ZhouL P Solomonson
May 9, 1995·Biochemistry·M J NelsonS P Seitz
May 30, 1995·Biochemistry·Q F GanE Sigal
Dec 20, 1994·Biochemistry·R C ScarrowM J Nelson
Apr 5, 1994·Biochemistry·M J SchilstraJ F Vliegenthart
Feb 1, 1994·Biochemistry·C J RocheD M Crothers
Jan 1, 1993·Progress in Nucleic Acid Research and Molecular Biology·C D Funk
Jun 4, 1993·Science·J C BoyingtonL M Amzel
Jun 29, 1993·Biochemistry·W MinorB Axelrod
Mar 1, 1996·The Journal of Biological Chemistry·K SchnurrH Kühn
Aug 15, 1996·The Biochemical Journal·R BrinckmannJ R Habenicht
Jul 8, 1996·FEBS Letters·R WiesnerH Kühn
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·H Kühn, B J Thiele
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·O Rådmark
Citations
Jul 27, 1999·Lipids·H KühnF Trebus
Jun 14, 2000·European Journal of Pharmacology·T YamamotoH Yamamoto
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Hartmut KuhnRalf Jürgen Kuban
May 8, 1999·Molecular Aspects of Medicine·J P EiserichV B O'Donnell
Jul 11, 2002·Free Radical Biology & Medicine·Hartmut Kühn, Astrid Borchert
Dec 1, 2011·Journal of Medicinal Chemistry·Mourad ChiouaAbdelouahid Samadi
Oct 13, 2000·British Journal of Pharmacology·S BastianettoR Quirion
Sep 12, 2009·Circulation Research·Volker Rudolph, Bruce A Freeman
Oct 5, 2010·European Journal of Medicinal Chemistry·Maria IspikoudiKonstantina C Fylaktakidou
Mar 15, 2006·Chemico-biological Interactions·Bao-Ming NieYang Lu
May 9, 2006·Progress in Lipid Research·Hartmut Kühn, Valerie B O'Donnell
Mar 1, 2012·The Biochemical Journal·Ashlee HigdonVictor M Darley-Usmar
Dec 27, 2011·Vascular Pharmacology·Nitin T AggarwalWilliam B Campbell
Oct 26, 2010·Progress in Lipid Research·Anca D DobrianJerry L Nadler
Dec 30, 2011·European Journal of Medicinal Chemistry·Krikor BijianMoulay A Alaoui-Jamali
Jan 5, 2001·Circulation Research·V B O'Donnell, B A Freeman
Dec 20, 2007·Journal of Cardiovascular Pharmacology·Hartmut KuhnMatthias Walther
Jan 5, 2002·Free Radical Research·M J CoffeyV B O'Donnell
Jul 10, 1999·The Journal of Biological Chemistry·V B O'DonnellB A Freeman
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M J CoffeyM Peters-Golden
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K SchnurrH Kuhn
Jul 1, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D C GoodwinL J Marnett
Oct 29, 2000·The Journal of Biological Chemistry·P K WittingA G Mauk
Nov 26, 2018·Progress in Lipid Research·Nikhlesh K Singh, Gadiparthi N Rao
Sep 28, 2005·Bioorganic & Medicinal Chemistry·George N ZiakasPanos N Kourounakis
Feb 5, 2019·Journal of Medicinal Chemistry·Mourad ChiouaJosé Marco-Contelles