PMID: 9182996May 1, 1997Paper

A kinetic model for the interaction of nitric oxide with a mammalian lipoxygenase

European Journal of Biochemistry
H G HolzhütterH Kühn

Abstract

Nitric oxide (NO) has been known for a long while to act as an inactivator of the soybean lipoxygenase-1 and cyclooxygenase. More recently, NO was shown to interact also with a mammalian 15-lipoxygenase [Wiesner, R., Rathmann, J., Holzhütter, H. G., Stosser, R., Mader, K., Nolting, H. & Kühn, H. (1996) Nitric oxide oxidises ferrous mammalian lipoxygenases to a pre-activated ferric species, FEBS Lett. 389, 229-232]. In this paper we present a detailed kinetic analysis of the interaction of NO with the 15-lipoxygenase from reticulocytes. Time courses of hydroperoxide formation were monitored after an anaerobic incubation of the enzyme with varying concentrations of NO and for varying length of the incubation period. Owing to the presence of O2 during the enzymatic reaction, the inhibitory effect of NO declines in a time-dependent manner since NO is converted into non-reactive NO2. This is manifested as a lag phase of the time course. The lag phase becomes more pronounced if the enzyme is incubated over a fixed period (15 s) with increasing concentrations of NO. In contrast, increasing the length of the incubation period at fixed concentrations of NO diminishes the duration of the lag phase. These experimental findings can be acco...Continue Reading

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Citations

Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Hartmut KuhnRalf Jürgen Kuban
Jul 11, 2002·Free Radical Biology & Medicine·Hartmut Kühn, Astrid Borchert
Sep 12, 2009·Circulation Research·Volker Rudolph, Bruce A Freeman
Oct 5, 2010·European Journal of Medicinal Chemistry·Maria IspikoudiKonstantina C Fylaktakidou
May 9, 2006·Progress in Lipid Research·Hartmut Kühn, Valerie B O'Donnell
Mar 1, 2012·The Biochemical Journal·Ashlee HigdonVictor M Darley-Usmar
Oct 26, 2010·Progress in Lipid Research·Anca D DobrianJerry L Nadler
Jul 10, 1999·The Journal of Biological Chemistry·V B O'DonnellB A Freeman
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M J CoffeyM Peters-Golden
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K SchnurrH Kuhn
Jul 1, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D C GoodwinL J Marnett
Nov 26, 2018·Progress in Lipid Research·Nikhlesh K Singh, Gadiparthi N Rao
Sep 28, 2005·Bioorganic & Medicinal Chemistry·George N ZiakasPanos N Kourounakis

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