PMID: 3760875Nov 1, 1986Paper

A kinetic study of stimulus-induced vesicle recycling in electromotor nerve terminals using labile and stable vesicle markers

Journal of Neurochemistry
D V AgostonT Yamaguchi

Abstract

The kinetics of recovery, by recycling electromotor synaptic vesicles, of the biophysical parameters of the reserve population has been studied in perfused blocks of electric organ of Torpedo marmorata prestimulated in vivo, followed by density gradient separation of the extracted vesicles in a zonal rotor using labile (acetylcholine and ATP) and stable (proteoglycan) vesicle markers. Stimulation in vivo at 0.15 Hz for 3.3 h depleted tissue acetylcholine much less than stimulation at 1 Hz for 1 h but nevertheless generated a much larger pool of recycled vesicles that recovered more slowly. At the lower rate of stimulation, recovery of the biophysical characteristics of the reserve population by the recycled vesicles, identified by their content of newly synthesized transmitter, was essentially complete by 8 h. The stable proteoglycan marker was immunochemically assayed and was bimodally distributed in the vesicle-containing portion of the density gradient even in experiments with unstimulated or recovered tissue. The second peak corresponded with that of newly synthesized transmitter and was thus identified as containing the recycled vesicles. Its normalized acetylcholine/proteoglycan ratio was lower than that of the first peak...Continue Reading

References

Jun 1, 1978·Journal of Neurochemistry·J B SuszkiwV P Whittaker
Sep 22, 1978·Brain Research·H Stadler, V P Whittaker
Oct 1, 1985·Journal of Neurochemistry·J W Kosh, V P Whittaker
Apr 1, 1974·The Biochemical Journal·M J DowdallV P Whittaker
Sep 1, 1968·The Biochemical Journal·F Fonnum
Jul 1, 1983·Journal of Neurochemistry·J H WalkerC W Zimmermann
Aug 1, 1984·Biochemical Society Transactions·V P Whittaker
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon

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Citations

Nov 1, 1995·Neurochemical Research·V P Whittaker, S Kelić

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