PMID: 2508631Sep 1, 1989Paper

A kinetic study of the suicide inactivation of an enzyme measured through coupling reactions. Application to the suicide inactivation of tyrosinase

The Biochemical Journal
J EscribanoF García-Cánovas

Abstract

A systematic procedure for the kinetic study of reaction mechanisms with enzyme inactivation induced by a suicide substrate in the presence or in the absence of an auxiliary substrate, when the enzyme activity is measured through coupling reactions, enzymically catalysed or not, was developed and analysed by using the transient-phase approach. The methodology is established to determine the parameters and kinetic constants corresponding to the enzyme suicide inactivation and the coupling reactions. This approach is illustrated by a study of the suicide inactivation of tyrosinase by catechol in the presence of L-proline. Treatment of the experimental data was carried out by non-linear regression.

Citations

Mar 11, 2010·Biochimica Et Biophysica Acta·J L Muñoz-MuñozJ N Rodríguez-López
Jul 31, 2013·IUBMB Life·Jose Luis Muñoz-MuñozFrancisco Garcia-Canovas
Mar 1, 2014·IUBMB Life·Maria del Mar Garcia-MolinaFrancisco Garcia-Canovas
Feb 22, 2012·Biochimica Et Biophysica Acta·Jose Luis Muñoz-MuñozFrancisco Garcia-Canovas
Jul 5, 1996·Biotechnology and Bioengineering·W Q Sun, G F Payne
Sep 18, 2004·International Journal of Biological Macromolecules·A A SabouryB Farzami
Jan 1, 1990·Journal of Enzyme Inhibition·H U Demuth
Apr 13, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Jose Luis Muñoz-MuñozFrancisco García-Cánovas
Nov 15, 2001·The Journal of Biological Chemistry·Frederic H VaillancourtLindsay D Eltis
Jul 24, 2008·The Biochemical Journal·Jose L Muñoz-MuñozJose N Rodríguez-López
Aug 22, 2001·Biochimica Et Biophysica Acta·J N Rodríguez-LópezJ Tudela
Sep 27, 2015·International Journal of Biological Macromolecules·Patricia Pérez GalendeValery L Shnyrov
Feb 22, 1995·Biochimica Et Biophysica Acta·A Sánchez-FerrerF García-Carmona
Apr 13, 1994·Biochimica Et Biophysica Acta·M García MorenoF García Cánovas
Sep 16, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tsai-Mu ChengSimon J T Mao
Jun 8, 2012·Journal of Agricultural and Food Chemistry·Maria del Mar Garcia-MolinaFrancisco Garcia-Canovas
Feb 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Vassiliki PapadimitriouAristotelis Xenakis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.