A kinetic study on the Novozyme 435-catalyzed esterification of free fatty acids with octanol to produce octyl esters

Biotechnology Progress
Avisha Chowdhury, Debarati Mitra

Abstract

Octyl esters can serve as an important class of biolubricant components replacing their mineral oil counterparts. The purpose of the current work was to investigate the enzymatic esterification reaction of free fatty acids (FFA, from waste cooking oil) with octanol in a solvent-free system using a commercial lipase Novozyme 435. It was found that the esterificaton reaction followed the Ping-pong bi-bi kinetics with no inhibition by substrates or products within the studied concentration range. The maximum reaction rate was estimated to be 0.041 mol L(-1) g(-1) h(-1) . Additionally, the stability of Novozyme 435 in the current reaction system was studied by determining its activity and final conversion of FFA to esters after 12 successive utilizations. Novozyme 435 exhibited almost 100% enzyme activity up to 7 cycles of reaction and gradually decreased (by 5%) thereafter. The kinetic parameters evaluated from the study shall assist in the design of reactors for large-scale production of octyl esters from a cheap biomass source. The enzyme reusability data can further facilitate mass production by curtailing the cost of expensive enzyme consumption.

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Citations

Nov 1, 2017·Biotechnology Progress·Nipon SarmahC Sumana
Jan 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·José Renato GuimarãesPaulo Waldir Tardioli
Apr 18, 2019·Biomacromolecules·Fatemeh ZabihiRainer Haag

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