A kinetic study on the phenothiazine dependent oxidation of NADH by bovine ceruloplasmin

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Rolf A Løvstad

Abstract

Tranquillizing drugs of the phenothiazine class form charge-transfer complexes with a ceruloplasmin-Cu(II) ion [De Mol NJ. 1985 Biochim Pharmacol 34, 2605-2609], the interaction resulting in a stimulatory effect on the ceruloplasmin catalyzed oxidation of catecholamines and NADH; the latter used as substrate in the present study. A good correlation between stability of the enzyme-drug complex and electron donor ability of the phenothiazine molecule was obtained for drugs with an aliphatic propyl side chain in 10-position (promazine > chlorpromazine > triflupromazine). The hydrofobic methyl group in the side chain of levomepromazine appeared to reduce the stability. A simple correlation between specific efficiency of the enzyme-drug complex and electron donor ability was not obtained (chlorpromazine > promazine = levomepromazine > triflupromazine). The Km-values, characterizing the reaction between NADH and the different enzyme-drug complexes, were estimated. The data suggest that the enzyme-chlorpromazine complex has the best affinity for NADH. The stimulatory effect of levomepromazine closely followed that of promazine.

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Citations

Jul 25, 2012·Free Radical Biology & Medicine·Douglas GaniniMaria B Kadiiska

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