A label free aptasensor for Ochratoxin A detection in cocoa beans: An application to chocolate industries

Analytica Chimica Acta
Rupesh K MishraJean-Louis Marty

Abstract

Contamination of food by mycotoxin occurs in minute/trace quantities. Nearly 92.5% of the cocoa samples present Ochratoxin A (OTA) levels at trace quantity. Hence, there is a necessity for a highly sensitive and selective device that can detect and quantify these organic toxins in various matrices such as cocoa beans. This work reports for the first time, a facile and label-free electrochemical impedimetric aptasensor for rapid detection and quantitation of OTA in cocoa beans. The developed aptasensor was constructed based on the diazonium-coupling reaction mechanism for the immobilization of anti-OTA-aptamer on screen printed carbon electrodes (SPCEs). The aptasensor exhibited a very good limit of detection (LOD) as low as 0.15 ng/mL, with added advantages of good selectivity and reproducibility. The increase in electron transfer resistance was linearly proportional to the OTA concentration in the range 0.15-2.5 ng/mL, with an acceptable recovery percentage (91-95%, RSD = 4.8%) obtained in cocoa samples. This work can facilitate a general model for the detection of OTA in cocoa beans based on the impedimetric aptasensor. The analysis can be performed onsite with pre-constructed and aptamer modified electrodes employing a porta...Continue Reading

References

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Citations

Dec 22, 2016·Sensors·Amina RhouatiJean-Louis Marty
Dec 23, 2015·Toxins·Tai Hwan Ha
Mar 24, 2018·Biosensors·Geetesh Kumar MishraRupesh K Mishra
Jun 7, 2016·The Analyst·Nuo DuanZhouping Wang
Mar 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pramod K GuptaPratima R Solanki
Sep 12, 2018·Foods·Geetesh Kumar MishraRupesh Kumar Mishra
Nov 2, 2019·Biosensors·Paloma Yáñez-SedeñoJosé Manuel Pingarrón
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonella Curulli
Jul 23, 2021·ACS Biomaterials Science & Engineering·Elif Burcu AydınMustafa Kemal Sezgintürk

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