A label-free fluorescent aptasensor for detection of kanamycin based on dsDNA-capped mesoporous silica nanoparticles and Rhodamine B

Analytica Chimica Acta
Shahrzad DehghaniSeyed Mohammad Taghdisi

Abstract

Kanamycin is an aminoglycoside antibiotic that can be useful against both gram negative and positive bacteria. However, if its serum levels are not controlled properly, it can cause serious side effects like ototoxicity and nephrotoxicity. The aim of this study was to design a simple and rapid fluorescent aptasensor for detection of kanamycin, based on Aptamer/Complementary strand (dsDNA)-capped mesoporous silica nanoparticles (MSNs) and Rhodamine B as a fluorescent probe. The MSNs pores were filled with Rhodamine B and then gated with dsDNA. In the presence of kanamycin, the aptamer sequence was separated from its complementary strand (CS), so that, uncovered the pores and leading to leakage of Rhodamine B. Thus, a significant increase in the fluorescence intensity was observed. The relative fluorescence intensity showed a linearity range from 24.75 nM to 137.15 nM of kanamycin with a detection limit of 7.5 nM. The aptasensor also showed to be useful for detection of kanamycin in serum samples and was able to distinguish kanamycin from other antibiotics, resulting in a sensitive, rapid and inexpensive method for kanamycin detection.

References

Nov 21, 1998·The EMBO Journal·S YoshizawaJ D Puglisi
Jul 28, 2012·Critical Care : the Official Journal of the Critical Care Forum·Martin SchulzAchim Schmoldt
Aug 15, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaoxiao HeJinlu Tang
Jan 9, 2013·Chemical Communications : Chem Comm·Frank J HernandezVeli C Özalp
May 11, 2013·Chemical Communications : Chem Comm·Mar OrovalPedro Amorós
Nov 5, 2014·Surface Science Reports·Alexander LibermanAndrew C Kummel
Apr 22, 2015·Chemical Communications : Chem Comm·M KavrukM Y Arica
Jan 6, 2016·Chemical Reviews·Elena AznarFélix Sancenón
Nov 4, 2016·Nature Reviews. Drug Discovery·Jiehua Zhou, John Rossi
Feb 22, 2017·ACS Applied Materials & Interfaces·Mar OrovalFélix Sancenón
Apr 30, 2017·Nature Reviews. Drug Discovery·Jiehua Zhou, John Rossi
Aug 24, 2017·Drug Development and Industrial Pharmacy·Mohammad Yahya Hanafi-BojdBizhan Malaekeh-Nikouei
Nov 17, 2017·Toxins·Amina RhouatiJean Louis Marty

❮ Previous
Next ❯

Citations

Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristina ChircovȘtefan Claudiu Turculeƫ
Jun 11, 2020·Critical Reviews in Analytical Chemistry·Elnaz BagheriMona Alibolandi
Oct 31, 2020·The Science of the Total Environment·Fengling YueXia Sun
Apr 22, 2019·Analytica Chimica Acta·Morteza Alinezhad NameghiSeyed Mohammad Taghdisi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.