A label-free immunosensor based on PtPd NCs@MoS2 nanoenzymes for hepatitis B surface antigen detection

Biosensors & Bioelectronics
Zhaoling TanYunhui Dong

Abstract

Nowadays, nanomaterials with enzymatic properties have aroused wide interest because of their special advantages, such as catalytic activity, simple preparation method and high stability. We introduced new nanoenzymes to a label-free electrochemical immunosensor for Hepatitis B surface antigen (HBs Ag) detection. In this study, PtPd nanocubes@MoS2 nanoenzymes (PtPd NCs@MoS2) were prepared by loading PtPd nanocubes (PtPd NCs) on molybdenum disulfide nano-sheet (MoS2) through in situ redox polymerization. The prepared nanoenzymes exhibited enhanced peroxidase-like activity than separate MoS2 and PtPd NCs. The catalytic process of PtPd NCs@MoS2 is in agreement with the Michaelis-Menten kinetic equation. PtPd NCs@MoS2 were used for sensitive detection of HBs Ag, which is ascribed to their superior peroxidase activity, good conductivity and high specific surface area and synergistic amplification for current signals. Compared with the detection limit of colorimetric method (3.3 pg/mL), the electrochemical method (10.2 fg/mL) shows a lower detection limit and a wider linear range from 32 fg/mL to 100 ng/mL, so it is more suitable for quantitative analysis of Hepatitis B. In summary, the prepared immunosensor provides a better opportu...Continue Reading

References

Jun 29, 2002·Journal of Clinical Microbiology·Carla Osiowy
Sep 24, 2005·Clinical Chemistry·Rudolf M Lequin
Mar 9, 2011·Biosensors & Bioelectronics·Xian-Xiang WangQian-Ming Huang
May 13, 2018·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Hui-Han HuHwai-I Yang
Aug 7, 2018·ACS Applied Materials & Interfaces·Kyung Hee ChoJyongsik Jang
May 6, 2019·BMC Infectious Diseases·Jean Damascene MakuzaSabin Nsanzimana

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Citations

Feb 2, 2021·Colloids and Surfaces. B, Biointerfaces·Jiangang XuXiaoyu Mu
Jan 19, 2021·Journal of Electroanalytical Chemistry·Hüseyin Oğuzhan KayaSeda Nur Topkaya
Mar 18, 2021·TheScientificWorldJournal·Aulia A TyasAkhmad Sabarudin
Dec 7, 2021·Analytical Chemistry·Sirong LiHui Wei

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