A laccase from the medicinal mushroom Ganoderma lucidum

Applied Microbiology and Biotechnology
Hexiang Wang, T B Ng

Abstract

A protein demonstrating laccase activity and potent inhibitory activity towards human immunodeficiency virus (HIV)-1 reverse transcriptase (IC50 1.2 microM) was isolated from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum. The laccase had a novel N-terminal sequence and a molecular mass of 75 kDa, which is higher than the range (55-56 kDa) reported for most other mushroom laccases. It was isolated by sequential chromatography on DEAE-cellulose and Affi-gel blue gel and adsorption on Con A-Sepharose. Unlike some of the previously isolated laccases, it was adsorbed only on Con A-Sepharose. The enzyme required a pH of 3-5 and a temperature of 70 degrees C to exhibit maximal activity. Minimal activity was detected at pH 6 and 7. Activity was undetectable at pH 8 and 9 and after exposure to 100 degrees C for 10 min.

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Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Maria do Rosário FreixoJosé Maria Arteiro
Aug 27, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·D D HuT B Ng
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Dec 10, 2014·Current Microbiology·Tamilvendan ManavalanKlaus Heese
Feb 9, 2018·International Journal of Molecular Sciences·Wensong JinZonghua Wang
May 7, 2008·Mycopathologia·Chih-Ching ChienChing-Hui Tseng
Jun 14, 2018·Current Protein & Peptide Science·Rong ZhouFang Liu
Oct 30, 2019·Current Protein & Peptide Science·Elham MohitMohammad Ali Faramarzi
Jul 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiao-Jiao ZhangHua-Bin Li
May 21, 2021·Saudi Journal of Biological Sciences·Khuanjarat ChoengpanyaKiattawee Choowongkomon
Jul 2, 2021·Advances in Virology·Maria S Serna-ArbeláezWildeman Zapata

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