A Lactate Fermentation Mutant of Toxoplasma Stimulates Protective Immunity Against Acute and Chronic Toxoplasmosis

Frontiers in Immunology
Ningbo XiaBang Shen

Abstract

Toxoplasma gondii is an important zoonotic pathogen infecting one-third of the world's population and numerous animals, causing significant healthcare burden and socioeconomic problems. Vaccination is an efficient way to reduce global sero-prevalence, however, ideal vaccines are not yet available. We recently discovered that the Toxoplasma mutant lacking both lactate dehydrogenases LDH1 and LDH2 (Δldh) grew well in vitro but was unable to propagate in mice, making it a good live vaccine candidate. Here, we tested the protection efficacy of ME49 Δldh using a mouse model. Vaccinated mice were efficiently protected from the lethal challenge of a variety of wild-type strains, including type 1 strain RH, type 2 strain ME49, type 3 strain VEG, and a field isolate of Chinese 1. The protection efficacies of a single vaccination were nearly 100% for most cases and it worked well against the challenges of both tachyzoites and tissue cysts. Re-challenging parasites were unable to propagate in vaccinated mice, nor did they make tissue cysts. High levels of Toxoplasma-specific IgG were produced 30 days after immunization and stayed high during the whole tests (at least 125 days). However, passive immunization of naïve mice with sera from va...Continue Reading

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Citations

Dec 22, 2020·International Journal for Parasitology·Nicholas C SmithGiel G van Dooren
Dec 17, 2020·Frontiers in Cellular and Infection Microbiology·Marie-Noëlle MévélecIsabelle Dimier-Poisson
Aug 19, 2021·Parasitology Research·Yaqiong LiBang Shen
Sep 8, 2021·The Biochemical Journal·Alba LlibreAaron Scott

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Methods Mentioned

BETA
ELISA
PCR
Protein Assay

Software Mentioned

GraphPad
Living Image
Prism
IVIS Spectrum

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