A Langendorff-like system to quantify cardiac pump function in adult zebrafish

Disease Models & Mechanisms
Hong ZhangXiaolei Xu

Abstract

Zebrafish are increasingly used as a vertebrate model to study human cardiovascular disorders. Although heart structure and function are readily visualized in zebrafish embryos because of their optical transparency, the lack of effective tools for evaluating the hearts of older, nontransparent fish has been a major limiting factor. The recent development of high-frequency echocardiography has been an important advance for in vivo cardiac assessment, but it necessitates anesthesia and has limited ability to study acute interventions. We report the development of an alternative experimental ex vivo technique for quantifying heart size and function that resembles the Langendorff heart preparations that have been widely used in mammalian models. Dissected adult zebrafish hearts were perfused with a calcium-containing buffer, and a beat frequency was maintained with electrical stimulation. The impact of pacing frequency, flow rate and perfusate calcium concentration on ventricular performance (including end-diastolic and end-systolic volumes, ejection fraction, radial strain, and maximal velocities of shortening and relaxation) were evaluated and optimal conditions defined. We determined the effects of age on heart function in wild-...Continue Reading

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Citations

Oct 13, 2020·Frontiers in Physiology·Maedeh Bazmi, Ariel L Escobar
Jan 29, 2021·Journal of Cardiovascular Development and Disease·Celine F SantiagoDiane Fatkin
Oct 28, 2020·Jornal brasileiro de nefrologia : ʹorgão oficial de Sociedades Brasileira e Latino-Americana de Nefrologia·Rosilene M EliasRosa M A Moysés
Dec 18, 2020·Frontiers in Physiology·Yonghe DingXiaolei Xu
Mar 26, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoguang LuWei Wang
May 16, 2021·American Journal of Human Genetics·Lore PottieBert Callewaert

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Methods Mentioned

BETA
dissecting

Software Mentioned

SAS
JMP
MathWorks
Amscope
HFE
ImageJ
MATLAB

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