PMID: 9000419Feb 1, 1997Paper

A large-conductance voltage-dependent potassium channel in cultured pig articular chondrocytes

Pflügers Archiv : European journal of physiology
J W MozrzymasF Ruzzier

Abstract

The patch-clamp techniques were used to study voltage-dependent potassium channels in cultured pig articular chondrocytes. A predominant single-channel conductance of 125 pS was found. These channels were reversibly blocked by tetraethylammonium. In cell-attached patches, transient increases in the channel activity were observed, and defined as a switching between low and high activity modes (LAM and HAM). Open-time distributions could be described with two kinetics components (in LAM and HAM) having similar time constants (fast tau1 and slow tau2). In HAM, the area of the slow component was larger. The mean burst length was significantly longer in HAM than in LAM. In both modes, the burst-length distributions were fitted with a sum of three exponentials. In LAM and HAM, the time constants tau1 and tau2 were indistinguishable from those of the open-time distributions. The slowest time constant, tau3, was strongly voltage dependent, and was significantly longer in HAM than in LAM. In both LAM and HAM, the ensemble currents were characterised by a rapid rising phase followed by fast and profound inactivation. The activation kinetics were similar, but the inactivation was faster in HAM. In the outside-out configuration no evidence...Continue Reading

Citations

Mar 17, 2011·Nature Communications·Stephen R Williams, Christian Wozny
Oct 16, 2012·Channels·Ali MobasheriRichard Barrett-Jolley
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Rebecca LewisRichard Barrett-Jolley
Aug 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Arturo Ponce
Apr 2, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A MobasheriR Barrett-Jolley
Mar 23, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Zuzanna SiwyKristinka Ivanova
Apr 24, 2002·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·S MercikZ Siwy
Nov 26, 1997·Biochimica Et Biophysica Acta·M MartinaF Vittur

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