A large, switchable optical clearing skull window for cerebrovascular imaging

Theranostics
Chao ZhangDan Zhu

Abstract

Rationale: Intravital optical imaging is a significant method for investigating cerebrovascular structure and function. However, its imaging contrast and depth are limited by the turbid skull. Tissue optical clearing has a great potential for solving this problem. Our goal was to develop a transparent skull window, without performing a craniotomy, for use in assessing cerebrovascular structure and function. Methods: Skull optical clearing agents were topically applied to the skulls of mice to create a transparent window within 15 min. The clearing efficacy, repeatability, and safety of the skull window were then investigated. Results: Imaging through the optical clearing skull window enhanced both the contrast and the depth of intravital imaging. The skull window could be used on 2-8-month-old mice and could be expanded from regional to bi-hemispheric. In addition, the window could be repeatedly established without inducing observable inflammation and metabolic toxicity. Conclusion: We successfully developed an easy-to-handle, large, switchable, and safe optical clearing skull window. Combined with various optical imaging techniques, cerebrovascular structure and function can be observed through this optical clearing skull wind...Continue Reading

Citations

Jan 22, 2020·Journal of Receptor and Signal Transduction Research·Yonglan ZhangWei Wang
Sep 18, 2020·Journal of Anatomy·Ting TianXiaoguang Li
Jun 27, 2019·Lasers in Surgery and Medicine·Nami DavoodzadehGuillermo Aguilar
Dec 29, 2020·Journal of Neuroscience Research·Luis Exequiel IbarraLaura Natalia Milla Sanabria
Mar 16, 2021·IScience·Tingting YuDan Zhu
Sep 11, 2021·Vascular Pharmacology·Jingtan ZhuDan Zhu

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence imaging
confocal microscopy
imaging techniques

Software Mentioned

Image Composite Editor
SPSS
NIS
USOCA

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here