A laser ablation method for the spatial segregation of enzyme and redox sites on carbon fiber microelectrodes

Analytical Chemistry
S E RosenwaldW G Kuhr

Abstract

A laser-generated interference pattern was used to remove enzyme from micrometer-wide stripes on an enzyme-covered carbon fiber microelectrode surface to create regions of facile electron transfer. Fluorescence microscopy was used to visualize fluorophore-tagged enzyme to indicate where the adsorbed enzyme remained on the surface. The electrochemical kinetics of the carbon fiber surface were examined to see if electron-transfer sites could indeed be segregated from enzyme adsorbed across the entire surface. CCD imaging of the electrochemical luminescence of Ru(bpy)3(2+) was used to verify the segregation between photoablated sites (with facile electron-transfer kinetics) and surfaces with adsorbed enzyme (which exhibit slow electron-transfer kinetics). The laser-ablated surface could also be distinguished from the enzyme-covered carbon surface with atomic force microscopy. Thus, photoablation of the surface of a protein-covered carbon fiber microelectrode with an interference pattern generated by a Nd:YAG laser allows the activation of 1.7-micron-wide bands of the electrode surface (available for facile electron transfer) while leaving 2.6-micron-wide enzyme-modified areas intact, thereby producing electroactive regions directl...Continue Reading

Citations

May 8, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·F GeD O Wipf
Feb 8, 2007·Angewandte Chemie·Gunther WittstockChuan Zhao
May 15, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ligia Maria MorettoPaolo Ugo
Sep 29, 2000·Analytical Chemistry·J L AndersonJ Leddy

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