A latex agglutination assay for specific detection of Panton-Valentine leukocidin

Journal of Microbiological Methods
Kanenari OishiKeiichi Hiramatsu

Abstract

Panton-Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenic potential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL. By testing 64 S. aureus strains, the assay could detect the 35 pvl-gene-positive strains with 100% specificity and sensitivity. Furthermore, the assay revealed an extensive variation in the amount of PVL produced by the pvl-positive strains.

References

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Jan 15, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stephanie M HamiltonDennis L Stevens

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Citations

Nov 5, 2013·Revue de pneumologie clinique·F ValourT Ferry
Oct 22, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Kristin A RareyRegis P Kowalski
Jul 8, 2011·International Journal of Antimicrobial Agents·Y GilletG Lina
May 5, 2009·International Journal of Antimicrobial Agents·Fadi BittarJean-Marc Rolain
Feb 13, 2014·Journal of Food Science·Yong WangChaonan Zhang

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