A Leaderless Two-Peptide Bacteriocin, Enterocin DD14, Is Involved in Its Own Self-Immunity: Evidence and Insights

Frontiers in Bioengineering and Biotechnology
Rabia LadjouziDjamel Drider

Abstract

Enterocin DD14 (EntDD14) is a two-peptide leaderless bacteriocin produced by Enterococcus faecalis 14, a strain previously isolated from meconium. EntDD14 has a strong antibacterial activity against Gram-positive bacteria. Leaderless bacteriocins, unlike bacteriocins with leader peptides, are immediately active after their translation, and a producing strain has then to develop specific mechanisms to protect both intra and extracellular compartments. The in silico analysis of Ent. faecalis 14 genome allowed to locate downstream of structural ddAB genes, 8 other adjacent genes, designed ddCDEFGHIJ, which collectively may form three operons. To gain insights on immunity mechanisms of Ent. faecalis 14, mutant strains knocked out in ddAB genes encoding bacteriocin precursor peptides (Δbac) and/or ABC transporter (ΔddI) of EntDD14 were constructed and characterized. Importantly, Δbac mutant strains, from which structural ddAB genes were deleted, resulted unable to produce EntDD14 and sensitive to exogenous EntDD14 showing their involvement in the Ent. faecalis 14 immunity system. Moreover, the sensitivity of Δbac mutants appeared not to be associated with the down-regulation of ddCDEFGHIJ gene expression since they were similarly ex...Continue Reading

References

Sep 1, 1989·Antimicrobial Agents and Chemotherapy·D F SahmB Clarke
Aug 1, 1980·Journal of Bacteriology·Y Yagi, D B Clewell
Mar 1, 1995·Applied and Environmental Microbiology·K Siegers, K D Entian
Aug 1, 1994·Applied and Environmental Microbiology·C Klein, K D Entian
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Oct 1, 1996·Antonie van Leeuwenhoek·I F NesH Holo
Jun 11, 2002·Journal of Molecular Biology·Daili Jacqueline Aguilar NetzHans-Georg Sahl
Feb 7, 2003·Applied and Environmental Microbiology·Marta DiazMercedes Maqueda
Mar 7, 2003·Applied and Environmental Microbiology·Marina Sánchez-HidalgoManuel Martínez-Bueno
May 4, 2005·Cellular and Molecular Life Sciences : CMLS·N Papo, Y Shai
Jun 28, 2005·Nucleic Acids Research·Cei Abreu-Goodger, Enrique Merino
Jun 6, 2006·Applied and Environmental Microbiology·Antonio M Martín-PlateroManuel Martínez-Bueno
Nov 6, 2007·Journal of Bacteriology·Matilde FernándezMercedes Maqueda
Jan 20, 2009·Current Pharmaceutical Biotechnology·G Bierbaum, H-G Sahl
Feb 16, 2010·Journal of Bacteriology·Morten KjosDzung B Diep
Apr 8, 2011·Journal of Agricultural and Food Chemistry·Xiaoji LiuMarco J van Belkum
Apr 28, 2011·Expert Opinion on Drug Metabolism & Toxicology·Auke Johan van HeelOscar P Kuipers
Dec 14, 2011·Journal of Bacteriology·Janaína Dos Santos NascimentoMaria do Carmo de Freire Bastos
Dec 22, 2012·Letters in Applied Microbiology·Y BelguesmiaJ-M Chobert
Dec 27, 2012·Nature Reviews. Microbiology·Paul D CotterColin Hill
Jan 22, 2013·Applied and Environmental Microbiology·Shun IwataniKenji Sonomoto
Jul 23, 2013·Biochemistry·André AbtsLutz Schmitt
Nov 19, 2013·Research in Microbiology·Marcus Lívio Varella CoelhoMaria do Carmo de Freire Bastos
Aug 5, 2014·Journal of Applied Microbiology·V M QuintanaM Chikindas
Sep 5, 2014·Microbial Cell Factories·Rodney H PerezKenji Sonomoto
Nov 20, 2014·Microbiology·Maria do Carmo de Freire BastosOlinda Cabral da Silva Santos
Apr 18, 2015·Frontiers in Microbiology·Ahmed K Al AtyaDjamel Drider
Oct 16, 2015·The Journal of Biological Chemistry·Carolin HackerJens Wöhnert
Nov 3, 2015·Research in Microbiology·Marcus Lívio Varella CoelhoMaria do Carmo de Freire Bastos
Nov 6, 2015·Journal of Applied Microbiology·Yoshimitsu MasudaKenji Sonomoto
Feb 11, 2016·Applied Microbiology and Biotechnology·Patricia Alvarez-SieiroOscar P Kuipers
Jun 19, 2016·Applied and Environmental Microbiology·Kirill V OvchinnikovDzung B Diep

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Citations

Oct 30, 2020·International Journal of Environmental Research and Public Health·Hassan ZgheibYanath Belguesmia
Jun 19, 2021·World Journal of Microbiology & Biotechnology·Hitomi OmachiTakeshi Kobayashi

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Datasets Mentioned

BETA
GM17
CP021161
EF502034.2
CP022712.1
CP015884.1
SRCM103341
CP035137.1
CP042833.1
DQ198088.1
CP040877.1

Methods Mentioned

BETA
PCR
gel filtration
electrophoresis

Software Mentioned

RibEX
Basic Local Alignment Search Tool
TMHMM
BLAST
GenePix Pro
Softberry
SnapGenes
SnapGene®4
ARNold

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