A lectin (CfLec-2) aggregating Staphylococcus haemolyticus from scallop Chlamys farreri

Fish & Shellfish Immunology
Peilin ZhengKezhi Xing

Abstract

Lectins are a family of carbohydrate-recognition proteins which play crucial roles in innate immunity. In this study, a new lectin (CfLec-2) gene was cloned from Chlamys farreri by EST and RACE approaches. The full-length cDNA of CfLec-2 was composed of 708bp, encoding a typical long form carbohydrate-recognition domain of 130 residues. The deduced amino acid sequence showed high similarity to Brevican in Homo sapiens, C-type lectin-1 and lectin-2 in Anguilla japonica. The cDNA fragment encoding the mature peptide of CfLec-2 was recombined into plasmid pET-32a (+) and expressed in Escherichia coli Rosseta-Gami (DE3). The recombinant CfLec-2 (rCfLec-2) protein exhibited aggregative activity toward Staphylococcus haemolyticus, and the agglutination could be inhibited by d-mannose but not EDTA or d-galactose, indicating that CfLec-2 was a Ca2+ independent lectin. Moreover, rCfLec-2 could suppress the growth of E. coli TOP10F'. These results suggested that CfLec-2 was perhaps involved in the recognition and clearance of bacterial pathogens in scallop.

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Citations

Jun 14, 2012·Fish & Shellfish Immunology·Leilei WangLinsheng Song
Nov 1, 2011·Developmental and Comparative Immunology·Leilei WangLinsheng Song
Jul 20, 2010·Developmental and Comparative Immunology·Jialong YangLinsheng Song
Aug 4, 2009·Fish & Shellfish Immunology·Xian-Wei WangJin-Xing Wang
Mar 7, 2009·Fish & Shellfish Immunology·Huan ZhangLinsheng Song
Feb 3, 2009·Developmental and Comparative Immunology·Huan ZhangLimei Qiu
Feb 16, 2011·Archives of Insect Biochemistry and Physiology·Xiao-Wen ZhangJin-Xing Wang
Apr 14, 2016·Developmental and Comparative Immunology·You-Ting ZhuQun Wang
Dec 9, 2014·Fish & Shellfish Immunology·Irina V ChikalovetsPavel A Lukyanov
Mar 24, 2015·Fish & Shellfish Immunology·Linsheng SongMengqiang Wang
Jun 25, 2017·Developmental and Comparative Immunology·Lingling WangLinsheng Song
Mar 8, 2018·International Journal of Molecular Sciences·Weilin WangLinsheng Song
Nov 12, 2013·Molecular Biology Reports·Changkao MuChunlin Wang
Apr 15, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Emmanuelle Pales EspinosaBassem Allam

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