A lectin-like receptor on murine macrophage is involved in the recognition and phagocytosis of human red cells oxidized by phenylhydrazine

Biochemical Pharmacology
S HornN Bashan

Abstract

Phenylhydrazine (Phz) is a powerful hemolytic agent which has several effects on both normal and G6PD deficient red blood cells (RBCs). We have studied the mechanism of removal of Phz-damaged human RBCs by murine macrophages. Phagocytosis of Phz-treated RBCs was found to be 50 RBCs/100 mac as compared to 2 RBCs/100 mac of the controls. EGTA and sodium azide inhibited the phagocytosis, indicating a requirement for both calcium ions and energy. Incubation of macrophages with sugars such as D-galactose or D-mannose reduced phagocytosis of Phz-treated RBCs by up to 60%, indicating the involvement of a macrophage lectin-like receptor in the recognition of Phz-treated RBCs. The presence of serum in the phagocytosis assay did not affect either phagocytosis of Phz-treated RBCs or inhibition by sugars. beta-Galactosidase, but not neuraminidase, treatment of RBCs caused a significant inhibition in phagocytosis of Phz-treated RBCs. These results suggest that galactosyl residues are exposed on RBC membrane during oxidation, probably not as a result of desialization. We conclude that Phz-treated RBCs are detected as damaged cells mainly due to sugar changes on their membrane, which are directly recognized by lectin-like receptors on the mac...Continue Reading

References

Jul 1, 1988·Toxicology and Applied Pharmacology·M A Runge-MorrisR F Novak
Oct 1, 1986·Cell Biochemistry and Function·M MagnaniG Fornaini
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J VaysseD Aminoff
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L McEvoyR A Schlegel
Sep 14, 1987·Biochimica Et Biophysica Acta·M BeppuK Kikugawa

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Citations

Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·G R SambranoD Steinberg
Jan 1, 1995·European Journal of Clinical Investigation·S HornJ Gopas
Mar 29, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·M BonominiA Arduini
Jul 25, 2009·Basic & Clinical Pharmacology & Toxicology·Josef Berger

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