PMID: 3748821Aug 26, 1986Paper

A left-handed (Z) conformation of poly(dA-dC).poly(dG-dT) induced by polyamines

Nucleic Acids Research
T Thomas, R P Messner

Abstract

Blocks of potential Z-DNA forming alternating purine-pyrimidine (APP) sequences are widely dispersed in native DNAs. We have studied the effects of naturally occurring polyamines on the conformation of a synthetic APP sequence, poly(dA-dC).poly(dG-dT) by circular dichroism spectroscopy. In the presence of micromolar concentrations of spermidine (125 microM) and spermine (16 microM), this polymer undergoes B to Z transition in low ionic strength (2 mM Na+) buffers. The concentration of polyamines required for B to Z transition increases with Na+ in the buffer and a straight line is obtained on plotting ln[Na+] vs. ln [spermidine 3+]. However, at concentrations of polyamines higher than those necessary to induce B to Z transition, Z-DNA converts to psi-DNA, an ordered, twisted, tight packing arrangement of the double helix. These results suggest a pathway for the transient formation of Z-DNA segments in vivo by interaction of the ubiquitous polyamines with naturally occurring blocks of APP sequences.

References

Mar 5, 1979·Journal of Molecular Biology·E E MinyatA K Schyolkina
May 29, 1979·Biochemistry·R W Wilson, V A Bloomfield
May 6, 1986·Biochemistry·A Woisard, G V Fazakerley
Mar 22, 1972·Nature: New Biology·C F JordanJ H Venable
Jan 1, 1980·Annual Review of Biophysics and Bioengineering·I TinocoM F Maestre
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·D H Russell
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·R D WellsW Zacharias
Jan 1, 1984·Annual Review of Biochemistry·A RichA H Wang
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·B RevetA Niveleau
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·T M JovinF Eckstein
Feb 1, 1984·Biopolymers·Y A Shin, G L Eichhorn
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H HamadaT Kakunaga
Feb 1, 1983·The Journal of Clinical Investigation·E M LaferB D Stollar
Apr 1, 1983·Biopolymers·T J Thomas, V A Bloomfield
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D J PatelA Rich
Apr 30, 1982·Science·R E DickersonM L Kopka
Jul 1, 1982·Biopolymers·W H BraunlinM T Record

❮ Previous
Next ❯

Citations

Jun 1, 1991·Bio/technology·G Caetano-AnollésP M Gresshoff
Sep 16, 2010·Physical Chemistry Chemical Physics : PCCP·Tarita BiverElisa Turriani
Jul 1, 1991·Immunological Investigations·U B GunniaT J Thomas
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Mar 19, 2003·Critical Reviews in Biochemistry and Molecular Biology·Miguel Angel MedinaFrancisca Sánchez-Jiménez
Jul 10, 1995·FEBS Letters·R HasanR Ali
Mar 18, 2008·Brain Research Reviews·P VasudevarajuK S J Rao
Mar 1, 2006·Biophysical Chemistry·Mayank M Patel, Thomas J Anchordoquy
Sep 2, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·J Ricardo Arias-Gonzalez
Mar 14, 2018·Organic & Biomolecular Chemistry·V Vongsutilers, P M Gannett
May 22, 1991·Proceedings. Biological Sciences·I S HaworthW G Richards
Jun 1, 1988·Biopolymers·G E Plum, V A Bloomfield
Feb 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·M DeianaM Samoc
May 12, 2016·Amino Acids·T J ThomasThresia Thomas
Mar 23, 2021·Biotechnology and Bioengineering·Hannah Panlilio, Charles V Rice
Sep 7, 1988·Biochimica Et Biophysica Acta·M J McLean, R D Wells

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.